Our standard Negative control siPOOL is recommended to accompany any siPOOL experiment.
Positive control siPOOLs are validated siPOOLs that produce defined characteristics when introduced into cells, and are recommended to confirm successful transfection.
Rescue sequences are used for secondary validation of siPOOL-mediated gene knock-down.
Rescue sequences are codon-optimized, siPOOL-resistant target gene sequences that can be synthesized and expressed in your choice system to “rescue” the phenotype afflicted by the siPOOL. This is performed for further validation of gene function. Please note that only the sequence is provided and gene synthesis is not included.
Positive Control siPOOL 2
2 nmol positive control siPOOL
Pos. control siPOOLs are pre-validated siPOOLs that produce defined characteristics when introduced into cells, indicating successful transfection. Pos. control siPOOLs available include siPOOLs against human PLK1 (5347) and KIF11 (3832) which both produce mitotic arrest and observable cell death; human INCENP (3619) which produces enlarged nuclei observable under the microscope; and human GAPDH (2597) which produces no observable phenotype but exhibits strong decrease in GAPDH RNA levels verifiable by quantitative PCR. Pos. control siPOOLs against mouse Plk1 (18817) and Kif11 (16551) are also available. To specify which pos. control siPOOL you want, simply indicate your preference in the message box at the final order confirmation page.
PAGE purified, freeze dried.
Negative Control siPOOL 2
2 nmol negative control siPOOL
neg. control siPOOL has been designed to have no significant sequence similarity to mouse, rat and human sequences. neg. control siPOOL has been tested in multiple cell lines and shows no significant impact on cell proliferation, apoptosis or cell morphology.
PAGE purified, freeze dried.
Negative Control siPOOL 5
5 nmol negative control siPOOL
neg. control siPOOL has been designed to have no significant sequence similarity to mouse, rat and human sequences. neg. control siPOOL has been tested in multiple cell lines and shows no significant impact on cell proliferation, apoptosis or cell morphology.
PAGE purified, freeze dried.
Positive Control siPOOL 5
5 nmol positive control siPOOL
Pos. control siPOOLs are pre-validated siPOOLs that produce defined characteristics when introduced into cells, indicating successful transfection. Pos. control siPOOLs available include siPOOLs against human PLK1 (5347) and KIF11 (3832) which both produce mitotic arrest and observable cell death; human INCENP (3619) which produces enlarged nuclei observable under the microscope; and human GAPDH (2597) which produces no observable phenotype but exhibits strong decrease in GAPDH RNA levels verifiable by quantitative PCR. Pos. control siPOOLs against mouse Plk1 (18817) and Kif11 (16551) are also available. To specify which pos. control siPOOL you want, simply indicate your preference in the message box at the final order confirmation page.
PAGE purified, freeze dried.
Negative Control siPOOL 10
10 nmol negative control siPOOL
neg. control siPOOL has been designed to have no significant sequence similarity to mouse, rat and human sequences. neg. control siPOOL has been tested in multiple cell lines and shows no significant impact on cell proliferation, apoptosis or cell morphology.
PAGE purified, freeze dried.
Positive Control siPOOL 10
10 nmol positive control siPOOL
Pos. control siPOOLs are pre-validated siPOOLs that produce defined characteristics when introduced into cells, indicating successful transfection. Pos. control siPOOLs available include siPOOLs against human PLK1 (5347) and KIF11 (3832) which both produce mitotic arrest and observable cell death; human INCENP (3619) which produces enlarged nuclei observable under the microscope; and human GAPDH (2597) which produces no observable phenotype but exhibits strong decrease in GAPDH RNA levels verifiable by quantitative PCR. Pos. control siPOOLs against mouse Plk1 (18817) and Kif11 (16551) are also available. To specify which pos. control siPOOL you want, simply indicate your preference in the message box at the final order confirmation page.
PAGE purified, freeze dried.
Negative Control siPOOL 20
20 nmol negative control siPOOL
neg. control siPOOL has been designed to have no significant sequence similarity to mouse, rat and human sequences. neg. control siPOOL has been tested in multiple cell lines and shows no significant impact on cell proliferation, apoptosis or cell morphology.
PAGE purified, freeze dried.
Positive Control siPOOL 20
20 nmol positive control siPOOL
Pos. control siPOOLs are pre-validated siPOOLs that produce defined characteristics when introduced into cells, indicating successful transfection. Pos. control siPOOLs available include siPOOLs against human PLK1 (5347) and KIF11 (3832) which both produce mitotic arrest and observable cell death; human INCENP (3619) which produces enlarged nuclei observable under the microscope; and human GAPDH (2597) which produces no observable phenotype but exhibits strong decrease in GAPDH RNA levels verifiable by quantitative PCR. Pos. control siPOOLs against mouse Plk1 (18817) and Kif11 (16551) are also available. To specify which pos. control siPOOL you want, simply indicate your preference in the message box at the final order confirmation page.
PAGE purified, freeze dried.
Negative Control siPOOL 40
40 nmol negative control siPOOL
neg. control siPOOL has been designed to have no significant sequence similarity to mouse, rat and human sequences. neg. control siPOOL has been tested in multiple cell lines and shows no significant impact on cell proliferation, apoptosis or cell morphology.
PAGE purified, freeze dried.
Positive Control siPOOL 40
40 nmol positive control siPOOL
Pos. control siPOOLs are pre-validated siPOOLs that produce defined characteristics when introduced into cells, indicating successful transfection. Pos. control siPOOLs available include siPOOLs against human PLK1 (5347) and KIF11 (3832) which both produce mitotic arrest and observable cell death; human INCENP (3619) which produces enlarged nuclei observable under the microscope; and human GAPDH (2597) which produces no observable phenotype but exhibits strong decrease in GAPDH RNA levels verifiable by quantitative PCR. Pos. control siPOOLs against mouse Plk1 (18817) and Kif11 (16551) are also available. To specify which pos. control siPOOL you want, simply indicate your preference in the message box at the final order confirmation page.
PAGE purified, freeze dried.
raPOOLs
Negative control raPOOL against E. coli LacZ may be used in RNA affinity purification experiments in mammalian systems as a control for non-specific binding.
Positive control raPOOL against human MALAT1 has been previously validated to successfully enrich published protein and DNA targets and may be used to confirm successful RNA affinity purification. See FAQ for more info.
Negative Control raPOOL 2
2 nmol negative control raPOOL
Positive control against human MALAT1 (NCBI ID: 378938)
Sufficient for 100 reactions
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