Ribosomal RNA depletion for Human-Mouse-Rat
The Human-Mouse-Rat riboPOOL is a single solution for ribodepletion in human, mouse and rat models. It efficienlty removes undesired cytoplasmic (28S, 18S, 5.8S, and 5S rRNA) and mitochondrial rRNA (mt16S and mt12S) from both intact and degraded samples.
Performance
Figure 1 Bioanalyzer profiles of human, mouse and rat total (gray) and rRNA-depleted (orange) RNA samples. For each species, 1 μg of total RNA was ribodepleted with the Human-Mouse-Rat riboPOOL.
List of available Human-mouse-rat riboPOOLs
Different Human-mouse-rat riboPOOLs depending on the application and type of input RNA:
| riboPOOL Name | Application | Input |
|---|---|---|
| Human-Mouse-Rat riboPOOL | RNA sequencing | Total RNA (RIN > 7) |
| Human-Mouse-Rat degraded RNA riboPOOL | RNA sequencing | Total RNA (RIN < 7) |
| Human-Mouse-Rat Ribo-Seq riboPOOL | Ribosome Profiling | Isolated RPFs |
Latest publications using Human-Mouse-Rat riboPOOLs
Selective gene expression maintains human tRNA anticodon pools during differentiation
Gao et al. (2024) Nature Cell Biology
RNF14-dependent atypical ubiquitylation promotes translation-coupled resolution of RNA-protein crosslinks
Zhao et al. (2023) Molecular Cell
Integrated workflow for discovery of microprotein-coding small open reading frames
Cao et al. (2023) STAR Protocols