Pan-riboPOOLs

Pan-riboPOOLs represent a versatile single-step solution for rRNA depletion across different phylogenetic groups. Unlike Single-Species riboPOOLs, designed to target rRNAs of specific organisms, the use of Pan-riboPOOLs  is recommended whenever rRNAs of multiple species have to be simultaneously depleted.

Applications of Pan-riboPOOLs across diverse research fields:

Our Pan-riboPOOLs portfolio includes options for wide (Pan-Bacteria, Pan-Fungi and Pan-Plants among others) and more targeted (e.g., Pan-Mammal, Pan-Bird, Pan-Actinobacteria) taxonomic coverage. You can find all our available Pan-riboPOOLs here.

Pan-riboPOOLs portfolio (rRNA depletion for multiple species)

Pan-Actinobacteria
ID: 35

Pan-Archaea
ID: 27

Pan-Bacteria
ID: 26

Pan-Bird
ID: 32

Pan-Fungi
ID: 43

Pan-Mammal
ID: 41

Pan-Mussel
ID: 89

Pan-Plant
ID: 31

Pan-Prokaryote
ID: 03

Pan-Sponge
ID: 44

Blood parasite
ID: 51

Filamentous fungi
ID: 06

H/M/R
ID: 53

L. orbiculatus & L. aequizonata
ID: 17

Mouse/Rat
ID: 55

Seawater
ID: 68

How do Pan-riboPOOLs work?

Pan-riboPOOLs probes are specifically designed to hybridise to conserved regions of each targeted rRNA, thus ensuring uniform coverage and robust depletion rates across all species. The design workflow of Pan-riboPOOLS include three main steps:

Pan-riboPOOL Performance

The Pan-Bacteria riboPOOL efficiently depleted > 90% of rRNA across Bacteria. 

Figure 1 Remaining rRNA expression of different bacteria species after ribodepletion with Pan-Bacteria riboPOOL. Expression values shown are normalized against a non-depleted total RNA sample.

What customers say

“Our group works with halophilic archaea and the main challenge for prokaryotic transcriptomics is the low rRNA:mRNA ratio. siTOOLs Biotech has custom-design probes that target Haloferax volcanii (HVO) and the Pan-Archaea riboPOOL for ribodepletion in a broad spectrum of archaea. Both riboPOOLs efficiently depleted > 90% of rRNA across the different species of halophilic Archaea (Halobacterium salinarum, Haloferax mediterranei and Haloarcula hispanica). The use of rRNA removal kits, like riboPOOLs can greatly help facilitate rapid progress in understanding the transcriptional response of a wide diversity of archaea to their environment”.
 

Dr. Mar Martinez-Pastor (Senior Scientist, Schmid Lab, Duke University)
Publication: Pastor et al. (2022) Biomolecules