Pan-Dictyostelia riboPOOL
The Pan-Dictyostelia riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from dictyostelids RNA samples. The product is compatible with most downstream applications, including different next generation sequencing workflows (both short and long-read sequencing).
The Pan-Dictyostelia riboPOOL is optimized for high-quality and intact (RIN > 7) RNA samples.
The Pan-Dictyostelia riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | No |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Dictyostelia, also known as social amoebae, are a group of single-celled organisms that exhibit remarkable social behaviors. These organisms can live independently as single cells but aggregate to form multicellular structures when food is scarce. This transition from unicellular to multicellular states provides a unique model for studying cellular communication, differentiation, and development.
The Pan-Dictyostelia riboPOOL was designed using the rRNA sequences from these species as templates:
Dictyostelium giganteum
Dictyostelium discoideum
Cavenderia aureostipes
Polysphondylium violaceum
Benefits of using riboPOOLs for multiple species rRNA depletion
- Enzyme-free, fast & easy workflow
- Compatible with most downstream applications
- Wide range of input RNA amounts
- Possibility of combining with other riboPOOLs
- Compatible with lab automated workflows