Human-Mouse-Rat Ribo-Seq riboPOOL
The Human-Mouse-Rat Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from human, mouse and rat during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
For high-quality and intact (RIN > 7) RNA samples, see Human-Mouse-Rat riboPOOL and for depletion of rRNA in FFPE/degraded sample, see Human-Mouse-Rat degraded RNA riboPOOL.
The Human-Mouse-Rat riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
In humans, mice, and rats, Ribo-Seq studies provide valuable data elucidating the intricate mechanisms governing cellular processes, facilitating a deeper understanding of biological functions and disease pathways.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species