Human tRNA Ribo-Seq
tRNA depletion kit
Removes tRNA from human total RNA
The Human tRNA Ribo-Seq riboPOOL is designed to efficiently remove transfer RNA (tRNA) during Ribo-Seq experiments. It’s also fully compatible with total RNA-Seq samples, making it a versatile option for project including both Ribo-Seq and RNA-Seq.
Ribodepletion with riboPOOLs can be easily integrated into Ribo-Seq protocols without requiring modifications to established workflows.
Other available Human tRNA or rRNA depletion kits
Transfer RNAs (tRNAs) are essential molecules in human cells that play a critical role in translating genetic information into proteins. These small RNA molecules function as adaptors, matching specific amino acids to their corresponding codons on messenger RNA (mRNA) during protein synthesis.
Depletion of transfer RNA (tRNAs)
Transfer RNAs (tRNAs) can also constitute a significant fraction of Ribo-Seq samples, and are often the most abundant RNAs after ribodepletion. Therefore it is often beneficial to deplete tRNAs alongside rRNAs. The Human tRNA and Mouse tRNA Ribo-Seq riboPOOLs have been developed to deplete tRNAs from Ribo-Seq samples and further enrich for RPFs in Ribo-Seq samples. The tRNA riboPOOLs can be directly mixed with any Ribo-Seq riboPOOL, allowing for rapid simultaneous depletion of both rRNA and tRNAs.
Figure 1 Proportion of reads mapping to different RNA types in Ribo-Seq samples after depletion with Human Ribo-Seq riboPOOL only (left), and Human Ribo-Seq riboPOOL in combination with the Human tRNA riboPOOL (right).
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species