Danio rerio
rRNA depletion kits
Efficient ribodepletion for Zebrafish
Our Danio rerio riboPOOLs efficiently remove rRNA from Danio rerio RNA samples. The product is compatible with most downstream applications, including different NGS workflows (both short and long-read sequencing).
Available riboPOOL products and their recommended applications:
| Product | Input RNA Quality | Input RNA | Application |
|---|---|---|---|
| Intact RNA | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| Intact/degraded RNA | Any quality | 10 ng- 3 µg | Standard RNA-seq |
| Ribosome Profiling | Any quality | 10 ng- 3 µg | Ribo-seq |
| High Input RNA | High Quality (RIN>7) | 5 µg - 30 µg | Standard RNA-seq |
Zebrafish embryos are transparent, allowing real-time observation of development. Studies focus on the genetic and molecular control of embryogenesis, organ formation, and tissue regeneration.
Shop riboPOOLs by application
Intact RNA
riboPOOL for high-quality samples
Intact and Degraded
riboPOOL for any-quality samples
Ribosome Profiling
riboPOOL for ribo-seq applications
High Input
riboPOOL for large RNA quantities
Not finding what you are looking for?
All of our riboPOOLs can be customized or combined with different species to provide individual solutions that fit your experiment. Contact us at info@sitools.de for more information.
Sequences covered by Danio rerio riboPOOLs
| Sequence | Type | Intact RNA | Intact/Degraded RNA | Ribosome Profiling | High Input |
|---|---|---|---|---|---|
| 28S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ |
| 18S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ |
| 5.8S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ |
| 5S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ |
| 16S rRNA | Mitochondrial | ✓ | ✕ | ✕ | ✓ |
| 12S rRNA | Mitochondrial | ✓ | ✕ | ✕ | ✓ |
Performance
Figure 1 Bioanalyzer profiles of Danio rerio total RNA before and after depletion with the Danio rerio riboPOOL for intact RNA.