Danio rerio riboPOOL for degraded RNA
The Danio rerio degraded RNA riboPOOL is designed to efficiently deplete ribosomal RNA (rRNA) from Danio rerio (zebrafish) FFPE or degraded RNA samples.
FFPE/degraded riboPOOLs cover the entire rRNA sequence and are designed for heavily degraded RNA samples, with RIN values below 6.
For high-quality and intact (RIN > 7) RNA samples, see Danio rerio riboPOOL.
Working on ribosome profiling? Check our Danio rerio Ribo-Seq riboPOOL.
The Danio rerio degraded RNA riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Zebrafish are amenable to genetic manipulation, including transgenesis and CRISPR/Cas9-mediated genome editing. Researchers utilize these tools to introduce or knock out specific genes, unraveling their roles in development, disease, and other physiological processes.
Benefits of using riboPOOLs for rRNA removal
- Enzyme-free, fast & easy workflow
- Compatible with most downstream applications
- Wide range of input RNA amounts
- Possibility of combining with other riboPOOLs
- Compatible with lab automated workflows