Human-Mouse-Rat degraded RNA riboPOOL

The Human-Mouse-Rat degraded RNA riboPOOL is designed to efficiently deplete ribosomal RNA (rRNA) from human, mouse and rat FFPE or degraded RNA samples. 

FFPE/degraded riboPOOLs cover the entire rRNA sequence and are designed for heavily degraded RNA samples, with RIN values below 6.

 

The Human-Mouse-Rat riboPOOL covers the following sequences:

Sequence Type Covered
28S rRNA Cytosolic Yes
18S  rRNA Cytosolic Yes
5.8S  rRNA Cytosolic Yes
5S rRNA Cytosolic Yes
16S  rRNA Mitochondrial No
12S  rRNA Mitochondrial No

 

RNA sequencing of formalin-fixed paraffin-embedded (FFPE) or degraded RNA samples from humans, mice, and rats presents unique challenges due to RNA degradation. Optimizing workflows can enhance the reliability of RNA-seq data obtained from degraded RNA samples, facilitating valuable insights into gene expression, regulatory mechanisms, and disease pathways in these model organisms and humans.

Benefits

  • Enzyme-free, fast & easy workflow
  • Compatible with most downstream applications
  • Wide range of input RNA amounts
  • Possibility of combining with other riboPOOLs
  • Compatible with lab automated workflows

Available Formats

riboPOOLs kits with cleanUP module

riboPOOL Probes Only