Which ribodepletion method works best?
Not sure what rRNA depletion strategy suits your experiment best? Follow the guidelines in the tables below.
Which organism do you work with?
| Taxon | Poly-A Selection | Ribodepletion |
|---|---|---|
| Bacteria | No | Yes |
| Archaea | No | Yes |
| Eukaryotes | Yes | Yes |
What downstream application do you plan?
| Application | Poly-A Selection | Ribodepletion |
|---|---|---|
| Short read sequencing | Yes | Yes |
| Long read sequencing | Yes | Yes |
| Direct RNA sequencing | Yes | Yes |
| Ribo-Seq | No | Yes |
Is your RNA intact or degraded?
| RNA Quality | Poly-A Selection | Ribodepletion |
|---|---|---|
| Intact (RIN > 8) | Yes | Yes |
| Partially degraded (6 < RIN < 8) | No | Yes |
| Degraded (RIN < 6) | No | Yes |
What sequences are you interested in?
| RNA | Poly-A Selection | Ribodepletion |
|---|---|---|
| Polyadenylated mRNAs | Yes | Yes |
| Long non-coding RNAs | No | Yes |
| Non-polyadenylated mRNAs | No | Yes |