RiboCut Marker
RNA Marker for precise excision of ribosome-protected fragments. Consisting of three PAGE-purified RNA oligos (27, 29 and 32 nucleotides in length) with a phosphate at their 3’ end.
Designed for RPFs
Oligo size of 27, 29 and 32 nucleotides in length enables precise excision of Ribosome Protected Fragments
Wide Sample Spectrum
Compatible with Ribo-Seq samples of different species. Usable for any species RPFs on TBE-Urea polyacrylamide gels.
Ease of use
Simply combine with SYBR Gold Nucleic Acid Gel Stain
Minimize your RNA contaminants with the RiboCut Marker
The purification of ribosome-protected fragments (RPFs) represents one of the critical steps of the ribosome profiling (Ribo-Seq) workflow. RPFs purification is usually carried out via polyacrylamide gel electrophoresis, followed by the excision of fragments with length of ~30 nucleotides. Purification of RPFs has to be performed precisely, as inaccurate excisions can significantly increase the abundance cof ribosomal RNAs (rRNA) and other unwanted contaminants.
The RiboCut Marker consists of three PAGE-purified RNA oligos (27, 29 and 32 nucleotides in length) (Fig. 1) with a phosphate at their 3' end, designed to facilitate the excision of RPFs from denaturing polyacrylamide gels. The RiboCut Marker is compatible with Ribo-Seq samples derived from different species (e.g. human, mouse, rat, C.elegans, D. melanogaster).
Figure 1. RiboCut Marker (left lane) and RNA digested (right lane) on a 15% polyacrylamide denaturing gel. The orange rectangle correspond to the gel area where RPFs are found.
Available Format
The RiboCut Marker can be used for up to 100 lanes and includes:
| Description | Quantity | Storage |
|---|---|---|
| RNA Oligo Mix | 1 tube (dry) | -80°C |
| 2 x Loading Dye | 1 tube (275 µl) | -20°C |
