We drive RNA Research with Advanced Molecular Tools


Exceptional Gene Silencing

siPOOLs

  • Potent & specific RNAi Knockdown
  • 30 siRNAs in one siPOOL
  • Reliable phenotypes
siPOOLs are optimized pools of 30 selected siRNAs. The high complexity of siPOOL s minimizes off-target effects and guarantes a robust knockdown.

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rRNA Depletion For Any Species

riboPOOLs

  • ribosomal RNA depletion for any species
  • Efficient, bias-free & cost-effective
  • For standard RNA-Seq & special applications
riboPOOLs are complex biotinylated probe sets for subtractive hybridization with magnetic beads. Designed for single and multiple species riboPOOLs economizing RNA-Seq for any organism.

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Fast, Semi-automated Fractionation

siFractor

  • Simple & reproducible
  • Precise & semi-automated
  • For structural biology, exosome research and translatome studies
The siFractor is a game changer for the fractionation of centrifugated samples. The siFractor facilitates connection to most FPLC/HPLC and allows you to exploit the full set of advantages provided by these host systems.

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World-leading scientists publish with our products



Publications citing siPOOLs


Nucleic Acids Research
Kerstin Dörner et al. (2022) Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism 

Stem Cell Research & Therapy
Falke et al. (2022) Knockdown of the stem cell marker Musashi-1 inhibits endometrial cancer growth and sensitizes cells to radiation

Nature Chemical Biology
Lechner et al. (2022) Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target


Publications citing riboPOOLs


Biomolecules
Pastor et al. (2022) Comparative Analysis of rRNA Removal Methods for RNA-Seq Differential Expression in Halophilic Archaea

Scientific Reports
Wahl et al. (2022) Comparison of rRNA depletion methods for efficient bacterial mRNA sequencing

BMC Genomics 
Kim, I et al. (2019) Efficient depletion of ribosomal RNA for RNA sequencing in planarians  


See all publications citing siTOOLs Biotech products


Supporting Data




Customer Testimonials

What Our Customers Are Saying

Find out how we helped these customers with their RNA research



What Customers Say About riboPOOLs

riboPOOLs



"We are a group at the LMU in Munich working with sponges and their microbiomes. We always had problems finding a good solution to economically perform rRNA depletion for these complex holobionts and sequence their metatranscriptomes. siTOOLs Biotech has designed and produced a Pan-Sponge riboPOOL for ribodepletion in sponges. Since we can combine different siTOOLs Biotech riboPOOLs, we can co-deplete sponge and bacterial rRNAs in one shot without losing any important transcriptomic information. This has opened new possibilities to study sponge holobionts in a more integrated manner."

Dr. Sergio Vargas
Department für Geo- und Umweltwissenschaften, LMU Germany


"We have tried Thermo RiboMinus Plant Kit and riboPOOL probe targeting Pan-plant to remove rRNA. It is necessary to perform two rounds of rRNA depletion with Thermo RiboMinusTM Plant Kit. Only one round is needed for riboPOOL probe."

Y. Long
Institute of Plant and Food Science, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China


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What Customers Say About siPOOLs

siPOOLs



"In our group, siPOOLs by siTOOLs for specific and sustained gene knockdown are used for several years now. We have tested and worked with approx. 35-40 siPOOLs by now. siPOOLs are easy in handling, and mostly effective in transient specific gene knockdown experiments. We could not detect any side effects, and the available control (control siPOOL) has no side effects on function or gene expression that we can detect as compared to untransfected cells. As compared to other commercially available single siRNAs, siPOOLs are much more effective, therefore, we can use less amounts of siPOOLs, which is very cost-efficient. Moreover, xenograft tumor models demonstrated that specific gene knockdown is sustained, i.e. gene knockdown is longer as compared to single siRNAs. siPOOLs are easy in handling, we use Lipofectamine 2000 or RNAimax as described by the manufacturer. The client service offered by siTOOLs is excellent. We get advice and even hands-on support immediately whenever necessary."

Dr. Med. Peter Dietrich, Post-doctoral Scientist
Institute of Biochemistry Prof. Dr. Anja Katrin Bosserhoff Group FAU, Germany


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What Customers Say about Phenovault

Phenovault



"My group worked with siTOOLs to analyze several siRNA screening datasets. Using Phenovault we were able to extensively analyze on- and off-target effects and ultimately identify several interesting cancer targets and pathways. Considering the complexity of the siRNA activity, phenovault provide very valuable information enabling to fully capitalize on experimental data. I would definitively recommend Phenovault to all investigators involved with siRNA screening."

Jean Philippe Stephan, Director Center of Excellence Pharmacological Screening,
Compound Management and Biobanking, Servier Research Institute, France


More about Phenovault



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High complexity POOLing - The Pack Hunter Approach



Why do we pool siRNAs

Why do we pool siRNAs?


At siTOOLs Biotech, we develop complex pools of selected oligos.
Oligo complexity guarantees robust and high efficacy by cooperative activity of multiple probes of one target.

The low concentration of each oligo dilutes undesired side effects and yields high reagent specificity.

siPOOLs are a set of 30 selected siRNAs with diverse seed sequences to minimize off-target effects and improve silencing robustness (Hannus et al., 2014).
Obtain reliable results with one single pool avoiding redunant experiments with multiple single siRNAs.
Learn more about siPOOLs

riboPOOLs are highly complex sets of biotinylated probes. rRNA pulldown is strongly enhanced by multiple probes binding to each rRNA fragment.
Pan-riboPOOLs cover groups of species with proprietary combination of probes targeting conserved ribosomal regions.
rRNA depletion of strongly fragemented RNA samples is achieved by a probe set with maximum probe density.
Learn more about riboPOOLs

raPOOLs are custom designed complex probe sets to enrich RNAs of interest.
Probe complexity allows reliable pull down of native RNAs with their interacting partners.
For Capture-Seq requires affordable, highly complex probeset for multiple genes.
Learn more about raPOOLs


How siTOOLs Biotech pool siRNAs


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RNA Research Services



RNAi


Custom siPOOL library design


Obtain libraries precisely the way you need
  • Design based on custom target list
  • library formating to customer plate layout
  • free choice of siPOOL concetration, plate type and aliquots


RNAi screening


Assay development and high content screening
  • siPOOL library of choice
  • Assay development & image-based screening
  • data analysis and hit validation


siPOOL validation


RT-PCR based knockdown quantification
  • single siPOOL or combination cell line from siTOOL collection or customer provided
  • quantification of downstream affected pathway


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RNA-Seq


Custom riboPOOL design


Depletion of any abundant RNA
  • for any sequenced species
  • rRNA, abundant mRNA, small RNAs
  • single species or multiple species


RNA-Seq service


RNA-Seq library preparation, sequencing and bioinformatics analysis
  • sequencing of any RNA sample
  • customized rRNA depletion
  • tailored bioinformatics analysis


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Bioinformatics


Phenovault


Analysis Suite to make sense of RNAi & CRISPR screening data
  • Discover novel drug targets
  • Hit list quality assessment
  • Gene target evaluation


More about Phenovault



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Upcoming Events






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Latest from Blog


06

Sep
The Iron Law of RNAi Screening
This is the lead singer of a band called Iron Law. He looks like a researcher experiencing massive frustration after discovering what we call the Iron Law of RNAi Screening. This law states that in...

Latest News


09

Sept

New Caenorhabditis elegans riboPOOL!





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