raPOOL

Targeted Robust RNA Capture for Biochemical Analysis

The discovery that 98% of the human genome is non-(protein)coding has fueled extensive interest in the functions of non-coding RNA (ncRNA). Long non-coding RNAs (lncRNAs), classified as ncRNA > 200 nt, have been implicated in various physiological processes and disease pathogenesis. As lncRNAs interact with protein, DNA and RNA to exert their effects, biochemical interaction studies to resolve their binding partners can provide valuable insight into their function.

RNA antisense pools (raPOOLs) consist of 30 optimally-designed 3'-biotinylated DNA probes that enable specific and efficient isolation of target lncRNA (or mRNA) complexed with nucleic acids or proteins. Used with streptavidin-coated magnetic beads, raPOOLs have shown robust RNA enrichment (~100 fold) and were published to successfully isolate known and novel lncRNA-interacting proteins (Nötzold et al. 2017).

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