siFractor

siFractor

Fast, Semi-automated Fractionation from Gradient Centrifugation Tubes


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A game changer for any application involving the fractionation of centrifugated samples


Manual fractionation of samples contained in centrifugation tubes is laborious, and error-prone. Parameters such as light absorbance are typically monitored from the isolated fractions, thus limiting resolution. Hence, precise isolation of the molecules of interest from the centrifugation is hampered. Overcome these limitations with the siFractor.

Benefits of using the siFractor


Simple and Reproducible Simple and reproducible
Precise Semi-Automated Precise & semi-automated
Fast and Leak-Free Fast & leak-free

Applications of the siFractor


Benefits of siFractor on Structural Biology Structural biology: Isolation of protein complexes and RNPs
Benefits of siFractor on Exosome Research Exosome research: Purification of extracellular vesicles
Benefits of siFractor on Ribosome Profiling Translatome studies: Ribosome profiling (Ribo-Seq)

 


Introducing the siFractor: simple, reproducible, precise & semi-automated fractionation



 


Technical details of the siFractor

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siFractor Technical Information

  • Sample holder with high pressure seals allowing fractionation at high flow rates and high viscosity range
  • Sample holders and piercing units available for different tube diameters
  • Extra robust piercing unit and adjustable table engaging the sample tube
  • M6 threads compatible with most FPLC/HPLC systems

 


Publications using the siFractor:

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bioRxiv
Meindl et al. (2022) A rapid protocol for ribosome profiling of low input samples



Current Protocols
Pichler et al. (2023) Ribosome Profiling in the Model Diatom Thalassiosira pseudonana



 


For further information:

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What customers say about the siFractor


 
"Due to its robust construction, the siFractor is much easier to handle than the device that we previously used for gradient fractionation (ISCO system). The quality of the separation was also very good in our experiments. The big advantage is that you don't have to buy all the components, i.e. pump, UV lamp and fractionator, as with other fractionation systems, but can use all of these components from the Äkta. This not only saves a lot of money, but also space" 

Dr. Brigitte Pertschy  
Group Leader, Institute of Molecular Biosciences, University of Graz


 


Request more information about the siFractor

Please use the form below to request for a quote or ask for more information about the siFractor.
























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