Fast, Semi-automated Fractionation from Gradient Centrifugation Tubes

   Request a quote


A game changer for any application involving the fractionation of centrifugated samples

Manual fractionation of samples contained in centrifugation tubes is laborious, and error-prone. Parameters such as light absorbance are typically monitored from the isolated fractions, thus limiting resolution. Hence, precise isolation of the molecules of interest from the centrifugation is hampered. Overcome these limitations with the siFractor.

Benefits of using the siFractor

Simple and Reproducible Simple and reproducible
Precise Semi-Automated Precise & semi-automated
Fast and Leak-Free Fast & leak-free

Applications of the siFractor

Benefits of siFractor on Structural Biology Structural biology: Isolation of protein complexes and RNPs
Benefits of siFractor on Exosome Research Exosome research: Purification of extracellular vesicles
Benefits of siFractor on Ribosome Profiling Translatome studies: Ribosome profiling (Ribo-Seq)


Introducing the siFractor: simple, reproducible, precise & semi-automated fractionation


Technical details of the siFractor

purple line

siFractor Technical Information

  • Sample holder with high pressure seals allowing fractionation at high flow rates and high viscosity range
  • Sample holders and piercing units available for different tube diameters
  • Extra robust piercing unit and adjustable table engaging the sample tube
  • M6 threads compatible with most FPLC/HPLC systems


Publications using the siFractor:

purple line

Meindl et al. (2022) A rapid protocol for ribosome profiling of low input samples

Current Protocols
Pichler et al. (2023) Ribosome Profiling in the Model Diatom Thalassiosira pseudonana


For further information:

purple line


What customers say about the siFractor

"Due to its robust construction, the siFractor is much easier to handle than the device that we previously used for gradient fractionation (ISCO system). The quality of the separation was also very good in our experiments. The big advantage is that you don't have to buy all the components, i.e. pump, UV lamp and fractionator, as with other fractionation systems, but can use all of these components from the Äkta. This not only saves a lot of money, but also space" 

Dr. Brigitte Pertschy  
Group Leader, Institute of Molecular Biosciences, University of Graz


Request more information about the siFractor

Please use the form below to request for a quote or ask for more information about the siFractor.

Subscribe to Our Newsletter
Be the first to get siTOOLs Biotech's Latest Product News, Exclusive Promotions, Blog Posts, and Research Highlights. By clicking Subscribe, you agree to our Privacy Policy.

Sign up for our Newsletter here

Follow Us on Social Media

Follow siTOOLs Biotech on Twitter   Follow siTOOLs Biotech on LinkedIn   Follow siTOOLs Biotech on Facebook   Follow siTOOLs Biotech on Instagram   Follow siTOOLs Biotech on YouTube