01 Discover novel targets
Identify novel genes that impact phenotypes by seed-based analysis of RNAi screening data.
02 Assess quality of hit list
For a given screening dataset, uncover likely false positives and obtain predicted expression levels.
03 Evaluate siRNA reagents
Determine potential for siRNA off-targeting and obtain candidate off-target gene list .
04 Evaluate gene targets
Validate targets by retrospective analysis of gene disruption phenotypes produced in Phenovault screens.
05 Identify relevant gene combinations
Discover gene combinations that impart relevant phenotypes with siTOOLs' Merlin Analysis algorithm.
06 Counteract screening artefacts and siRNA off-targeting
Uncover screening artefacts (e.g. plate positional effects) and improve hit list with siRNA seed-based correction.