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Technotes



Technotes



Technote 1: siRNA Off-target Effects - Causes, Extent & Impact

 
Learn about siRNA off-target effects. A reference-rich guide describing the mechanism by which siRNA off-targets are produced, the penetrance and extent of siRNA off-target effects, and the impact siRNA off-targets have on experimental research.

Download Technote 1: siRNA Off-target Effects - Causes, Extent & Impact
 
Technote 1-170720 - siRNA off-target effects - Causes, extent & impact
 

Technote 2: Ways to Reduce siRNA Off-target Effects

 
Understand current established techniques to reduce siRNA off-target effects, including the siPOOL approach. From siRNA concentration, chemical modification and siRNA design, this is a detailed technical overview of each approach and their pros and cons. We also provide a non-bias assessment of the effectiveness of each approach using data-driven bioinformatic analysis.

Download Technote 2: Ways to Reduce siRNA Off-target Effects
 
Technote 2-181001 - Ways to Reduce siRNA Off-target Effects
 

Product Brochures


siTOOLs Product/Service Overview

A snapshot overview of our products and services.

Download the siTOOLs Overview
 
siTOOLs Product/Service Overview


The siPOOL Guide: The Pack Hunter Approach to Superior Gene Silencing.

A detailed guide with an introduction to the RNAi off-target problem and how siPOOLs were developed to counter them. Covers the three main features of siPOOLs - high complexity pooling, detailed bioinformatics-based design and quality production process - and the benefits they confer. Includes proof-of-concept data, customer testimonials and case study applications.

Download the siPOOL Guide
 
The siPOOL Guide - Pack Hunter Approach to Superior Gene Silencing, Clean, Reliable and Hassle-free RNAi


The siPOOL Human Kinase Library

siPOOLs are ideal for reliable high-throughput functional genomic screening. Learn more about the siPOOL human kinase library, the screening benefits it confers and associated data in this brochure.

Download siPOOL Human Kinase Library brochure
siPOOL Human Kinase Library


The siPOOL Cancer Toolbox

The siPOOL cancer toolbox is a useful exploratory tool for scientists seeking an easy method to disrupt key cancer genes singly or in combination.

Download siPOOL Cancer Toolbox brochure
 
siPOOL Cancer Toolbox


The Phenovault

The Phenovault is a free-access data analysis and reagent/gene evaluation tool that leverages new insights based on historical RNAi/CRISPR screening data. To schedule a presentation about Phenovault, please contact us.

Download The Phenovault brochure
Download The Phenovault poster
Phenovault


siPOOL-resistant rescue construct/sequence

To further validate that the loss-of-function phenotype produced by a siPOOL is specific to the targeted gene, we offer a siPOOL-resistant rescue sequence. When expressed in a plasmid construct, target gene function is restored which rescues/reverses the loss-of-function phenotype.

Download siPOOL-resistant rescue information.







Contact us to get the most out of your gene function studies.


+49 (0) 89 12501 4800            info@sitools.de




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