World Leading Scientist publish with riboPOOLs

Bioscience, Biotechnology, and Biochemistry
Mio Takeuchi, Hideyoshi Yoshioka (2021) Acetate excretion by a methanotroph, Methylocaldum marinum S8, under aerobic conditions

Nature Protocols
Y. Long (2021) FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing

JBC
Neil Fleck & Christoph Grundner (2021) A Cas12a-based CRISPR interference system for multigene regulation in mycobacteria

npj Biofilms and Microbiomes
J. Andersen et al. (2021) Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms

Nature Communications
La Rosa et al. (2021) Compensatory evolution of Pseudomonas aeruginosa’s slow growth phenotype suggests mechanisms of adaptation in cystic fibrosis 12 3186

 

PLOS Genetics
Fuchs et al. (2021) Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach

BMC Genomics 
Kim, I et al. (2019) Efficient depletion of ribosomal RNA for RNA sequencing in planarians 20, 909

 

Ribosome profiling

Science
Bhat et al. (2021) Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome

Nature Communications
Blaze et al. (2021) Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior, 587, 14

Nat Protoc 
Galmozzi, C. V. et al. (2019) Selective ribosome profiling to study interactions of translating ribosomes in yeast 14, 2279–2317

Nature Communications
Ryan. D et al. (2020) A high-resolution transcriptome map identifies small RNA regulation of metabolism in the gutmicrobe Bacteroides thetaiotaomicron (20) 11

             
 

What Customers Say:

Oxford Nanopore Sequencing

"We have tried Thermo RiboMinus Plant Kit and riboPOOL probe targeting Pan-plant to remove rRNA. It is necessary to perform two rounds of rRNA depletion with Thermo RiboMinusTM Plant Kit. Only one round is needed for riboPOOL probe." 
Y. Long (Institute of Plant and Food Science, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China)
 

Ribosome Profiling (Ribo-Seq)

"We are doing a lot of ribosome profiling experiments - mainly using mouse tissues and cell lines - and were in need of a ribosomal RNA depletion tool that was robust, economical, and adaptable.
Initially, we asked siTOOLs Biotech to design specific antisense probes against the most abundant rRNA contaminants that we tended to see in our ribosome profiling data, and that severely reduced the amount of usable reads.
The probes reduced the ribosomal RNAs they were designed for very well, however (and probably as to be expected) other rRNA contaminants subsequently became predominant and we needed a more uniform, rational tool that would cover all rRNA contaminants. 
Hence, siTOOLs Biotech designed and produced a ribosomal RNA depletion kit depleting both extremely abundant rRNA contaminants and less abundant ones.
We are very happy with the support, reactivity, and overall scientific expertise and knowledge of the siTOOLs team, which allowed us to have a great tool for a variety of experiments ongoing in the lab."
David GATFIELD (University of Lausanne, Center for Integrative Genomics (CIG)) Publication: Bhat et al. (Gatfield) (2021) Science