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riboPOOLs

riboPOOL

Affordable, Custom Ribosomal RNA Depletion for Any Species

Removing ribosomal RNAs (rRNAs) to allow sensitive detection of scientifically relevant RNAs by Next Generation Sequencing, is currently performed with costly kits limited to well-studied species.
riboPOOLs present an affordable and flexible solution that gives scientists absolute freedom to deplete rRNAs or other custom RNAs from any species.

Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs even outperformed Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.

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Advantages of Our Technology

01 Highly efficient and specific

Human and mouse riboPOOLs depleted > 95% cytoplasmic and mitochondrial rRNA.

02 Any species

Deplete rRNA from any species of your choice. Human, Mouse and Planarian in-stock. New species available on demand.

03 Low cost solution

A fraction of the price of existing solutions.

04 Deplete custom RNA

Deplete any RNA of your choice, e.g. globin from whole blood samples.

05 Applicable for non-polyadenylated RNA

riboPOOLs do not depend on polyA- enrichment enabling detection of non-polyadenylated RNA.

06 Custom scales available

Larger scales available for custom application.

Supporting Data

Specific, efficient rRNA depletion with riboPOOLs

Human riboPOOL depleted 98.6% of rRNA, compared to 80.6% by Ribo-Zero

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Mouse riboPOOL depleted 96.4% of rRNA, compared to 86.1% by Ribo-Zero

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Reproducible results with riboPOOLs

High reproducibility between biological replicates with human riboPOOLs

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High reproducibility between biological replicates with mouse riboPOOls

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HOW IT WORKS

riboPOOL Technology

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Contact us to get the most out of your gene function studies.

+49 (0) 89 12501 4800

info@sitools.de