riboPOOLs Special Applications
riboPOOLs can be customized to fit your RNA samples. We create riboPOOLs for any mixture of RNA (e.g., Seawater samples), strongly degraded RNA (i.e., FFPE samples), or ribosome profiling (Ribo-Seq) samples
FFPE samples & degraded RNA samples
Total RNA extracted from FFPE tissue (Formalin-fixed, paraffin-embedded tissues) are heavily degraded, with RIN values below 2.
Due to their strong fragmentation, ribosomal RNAs are difficult to remove before RNA sequencing (RNA-Seq).
With a gapless and tiled probe coverage of the entire rRNA sequence, siTOOLs Biotech developed an efficient rRNA depletion tool for FFPE and other strongly degraded RNA samples.
We currently have human, mouse/rat, Caenorhabditis elegans, and Drosophila melanogaster riboPOOLs for degraded RNA samples.
Ribosome Profiling (Ribo-Seq)
Ribosome Profiling (Ribo-Seq) identifies actively translated RNAs by sequencing only those 30-base mRNA fragments protected by ribosomes.
The nuclease digest, degrading unprotected mRNA outside of ribosomes, also generates highly abundant ribosomal RNA contaminations, making up many sequencing reads.
siTOOLs Biotech has generated an efficient Ribo-Seq riboPOOL targeting primarily those extremely abundant rRNA contaminants commonly found in Ribo-Seq samples.
The entire rRNA sequence is covered with capture probes to deplete any less abundant rRNA fragment.
Our Ribo-Seq riboPOOL portfolio includes rRNA removal solutions for human, mouse/rat, Drosophila melanogaster, and Caenorhabditis elegans Ribo-Seq samples.
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