What Customers Say:
“Our group works with halophilic archaea
and the main challenge for prokaryotic transcriptomics is the low rRNA:mRNA ratio. siTOOLs Biotech has custom-design probes that target Haloferax volcanii
(HVO) and the Pan-Archaea riboPOOL
for ribodepletion in a broad spectrum of archaea. Both riboPOOLs efficiently depleted > 90% of rRNA across the different species of halophilic Archaea (Halobacterium salinarum, Haloferax mediterranei and Haloarcula hispanica
). The use of rRNA removal kits, like riboPOOLS can greatly help facilitate rapid progress
in understanding the transcriptional response of a wide diversity of archaea to their environment”.
Dr. Mar Martinez-Pastor
(Senior Scientist, Schmid Lab, Duke University)
Publication: Pastor et al. (2022) Biomolecules
Oxford Nanopore Sequencing
"We have tried Thermo RiboMinus Plant Kit and riboPOOL probe targeting Pan-plant to remove rRNA. It is necessary to perform two rounds of rRNA depletion with Thermo RiboMinusTM Plant Kit. Only one round is needed for riboPOOL probe
(Institute of Plant and Food Science, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China)
Ribosome Profiling (Ribo-Seq)
"We are doing a lot of ribosome profiling
experiments - mainly using mouse tissues and cell lines - and were in need of a ribosomal RNA depletion tool that was robust, economical, and adaptable.
Initially, we asked siTOOLs Biotech to design specific antisense probes against the most abundant rRNA contaminants that we tended to see in our ribosome profiling data, and that severely reduced the amount of usable reads.
The probes reduced the ribosomal RNAs they were designed for very well, however (and probably as to be expected) other rRNA contaminants subsequently became predominant and we needed a more uniform, rational tool that would cover all rRNA contaminants.
Hence, siTOOLs Biotech designed and produced a ribosomal RNA depletion kit
depleting both extremely abundant rRNA contaminants and less abundant ones.
We are very happy with the support, reactivity, and overall scientific expertise and knowledge of the siTOOLs team, which allowed us to have a great tool for a variety of experiments ongoing in the lab."
(University of Lausanne, Center for Integrative Genomics (CIG)) Publication: Bhat et al. (Gatfield) (2021) Science