riboPOOL

Efficient, Affordable Ribosomal RNA Depletion for Any Species



Detection of scientifically relevant RNAs by Next-Generation RNA Sequencing (RNA-Seq) is greatly limited by highly abundant ribosomal RNAs (rRNAs) which occupy > 90% RNA-Seq reads. Current rRNA depletion solutions however are often costly and limited to well-studied species.

Ribosomal RNA depletion pools (riboPOOLs) by siTOOLs Biotech is the market's most flexible solution in rRNA depletion. With a diverse selection of Ready-Made riboPOOLs and the ability to create your own Custom riboPOOL, riboPOOLs are ideal for sequencing experts with custom demands and scientists working on rare species.

Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs are highly efficient, even outperforming Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.

For microbiologists, the new Pan-Prokaryote riboPOOL provides universal rRNA depletion for a wide spectrum of microbes and is suited for metagenomic or microbiome samples. Combination riboPOOLs are also available to deplete rRNAs from samples containing multiple species.


Product Information


riboPOOLs are HPLC-purified and shipped freeze-dried at room temperature.
riboPOOLs are available for diverse species. Please download our Ready-Made and Custom riboPOOL Brochure for full species list.
For greatest flexibility, riboPOOLs may be combined in custom ratios to form a Combination riboPOOL that can be used for samples across multiple species. Please download the riboPOOL Application Note for data on Combination riboPOOL (Human/Mouse/Pan-Prokaryote). Please request a quote or email us for pricing/ordering.

riboPOOLs are provided either as:

  1. Probes alone with nuclease-free water. Please refer to our protocol for other reagents required. riboPOOLs in this format are available for Quick Order via our Webshop.
  2. Complete riboPOOL kits that include buffers, streptavidin-magnetic beads and ethanol precipitation reagents. Delivery in mid-November. Please contact us or your local distributor for a quote.




Advantages of Our Technology



01 Highly efficient and specific

Human and mouse riboPOOLs depleted > 95% cytoplasmic and mitochondrial rRNA.

02 Any species

Deplete rRNA from any species of your choice. Diverse species in-stock. New species available on demand.

03 Low cost solution

A fraction of the price of existing solutions.

04 Deplete custom RNA

Deplete any RNA of your choice, e.g. globin from whole blood samples.

05 Applicable for non-polyadenylated RNA

riboPOOLs do not depend on polyA- enrichment enabling detection of non-polydenylated RNA.

06 Suitable for metagenomic applications

Pan-Prokaryote and Combination riboPOOLs available for samples containing multiple species.



Supporting Data




Specific, efficient rRNA depletion with riboPOOLs



Human riboPOOL depleted 98.6% of rRNA, compared to 80.6% by Ribo-Zero

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Mouse riboPOOL depleted 96.4% of rRNA, compared to 86.1% by Ribo-Zero

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Reproducible results with riboPOOLs



High reproducibility between biological replicates with human riboPOOLs

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High reproducibility between biological replicates with mouse riboPOOls

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HOW IT WORKS

rRNA Depletion workflow





Composed of a highly complex mixture of optimally-designed 3'-biotinylated DNA probes, riboPOOLs hybridize efficiently and specifically to ribosomal RNAs (rRNAs) present in total RNA.

Streptavidin-coated magnetic beads are added that allow efficient separation of riboPOOL-bound rRNAs through the high affinity biotin-streptavidin interaction.

Remaining relevant RNAs are purified and concentrated by either ethanol precipitation, spin-column purification or SPRI bead-based clean-up, before analysis by Next-Generation Sequencing, microarray or PCR.



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