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riboPOOL

Ribosomal RNA Depletion for Any Species



Ribosomal RNA depletion pools (riboPOOLs) by siTOOLs Biotech is the market's most flexible solution in rRNA depletion. With a diverse selection of Ready-Made riboPOOLs and the ability to create your own Custom riboPOOL, riboPOOLs are ideal for sequencing experts with custom demands and scientists working on rare species.



 

riboPOOL Selection Guide


single species
multiple species
other abundant RNA





For new species, a Custom riboPOOL can be made with a one-time custom riboPOOL setup service.

For increased flexibility, riboPOOLs may be combined in custom ratios to form a Combination riboPOOL that can be used for samples containing or across multiple species. See riboPOOL Application Note under Downloads for performance data.

riboPOOLs can be obtained by Quick Order, Quote Request or through your local distributor.

Available riboPOOL formats:

riboPOOL Kit

  • All reagents up to library preparation included
  • Manual provided
  • Reaction sizes: 12, 24, 96
    A 6 reaction trial kit is available for first-time purchase, please contact us for a quote.

riboPOOL Probes

  • Only riboPOOL probes and nuclease-free water provided
  • Protocol downloadable
  • Reaction sizes: 20 (2 nmol), 50 (5 nmol), 100 (10 nmol)



Find our webinar recording here:





Download


riboPOOL brochure
Ready-Made and Custom riboPOOL Brochure
Pan-Prokaryote riboPOOL Brochure
Pan-Plant riboPOOL Brochure
riboPOOL Poster
riboPOOL Application Note
riboPOOL Kit Manual
 


Advantages of Our Technology



01 Highly efficient and specific

Human and mouse riboPOOLs depleted > 95% cytoplasmic and mitochondrial rRNA.

02 Any species

Deplete rRNA from any species of your choice. Diverse species in-stock. New species available on demand.

03 Low cost solution

A fraction of the price of existing solutions.

04 Deplete custom RNA

Deplete any RNA of your choice, e.g. globin from whole blood samples.

05 Applicable for non-polyadenylated RNA

riboPOOLs do not depend on polyA- enrichment enabling detection of non-polydenylated RNA.

06 Suitable for metagenomic applications

Pan-Prokaryote and Combination riboPOOLs available for samples containing multiple species.



Supporting Data




Specific, efficient rRNA depletion with riboPOOLs



Human riboPOOL depleted 98.6% of rRNA, compared to 80.6% by Ribo-Zero

riboPOOL

Mouse riboPOOL depleted 96.4% of rRNA, compared to 86.1% by Ribo-Zero

riboPOOL


Reproducible results with riboPOOLs



High reproducibility between biological replicates with human riboPOOLs

riboPOOL

High reproducibility between biological replicates with mouse riboPOOls

riboPOOL



HOW IT WORKS

rRNA Depletion workflow





Composed of a highly complex mixture of optimally-designed 3'-biotinylated DNA probes, riboPOOLs hybridize efficiently and specifically to ribosomal RNAs (rRNAs) present in total RNA.

Streptavidin-coated magnetic beads are added that allow efficient separation of riboPOOL-bound rRNAs through the high affinity biotin-streptavidin interaction.

Remaining relevant RNAs are purified and concentrated by either ethanol precipitation, spin-column purification or SPRI bead-based clean-up, before analysis by Next-Generation Sequencing, microarray or PCR.





Product Information


riboPOOLs are HPLC-purified and shipped freeze-dried at room temperature.




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