Efficient, Affordable Ribosomal RNA Depletion for Any Species
Detection of scientifically relevant RNAs by Next-Generation RNA Sequencing (RNA-Seq) is greatly limited by highly abundant ribosomal RNAs (rRNAs) which occupy > 90% RNA-Seq reads. Current rRNA depletion solutions however are often costly and limited to well-studied species.
Ribosomal RNA depletion pools (riboPOOLs) by siTOOLs Biotech is the market's most flexible solution in rRNA depletion. With a diverse selection of Ready-Made riboPOOLs and the ability to create your own Custom riboPOOL, riboPOOLs are ideal for sequencing experts with custom demands and scientists working on rare species.
Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs are highly efficient, even outperforming Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.
For microbiologists, the new Pan-Prokaryote riboPOOL provides universal rRNA depletion for a wide spectrum of microbes and is suited for metagenomic or microbiome samples. Combination riboPOOLs are also available to deplete rRNAs from samples containing multiple species.
riboPOOLs are HPLC-purified and shipped freeze-dried at room temperature.
riboPOOLs are available for diverse species. Please download our Ready-Made and Custom riboPOOL Brochure for full species list.
For greatest flexibility, riboPOOLs may be combined in custom ratios to form a Combination riboPOOL that can be used for samples across multiple species. Please download the riboPOOL Application Note for data on Combination riboPOOL (Human/Mouse/Pan-Prokaryote). Please request a quote or email us for pricing/ordering.
riboPOOLs are provided either as:
- Probes alone with nuclease-free water. Please refer to our protocol for other reagents required.
- Complete riboPOOL kits that include buffers, streptavidin-magnetic beads and ethanol precipitation reagents.
riboPOOLs can be obtained by Quick Order, Quote Request or through your local distributor.