Ribosomal RNA depletion for any species and application

riboPOOLs consist of complex pools of biotinylated DNA probes, specifically designed to hybridize and remove ribosomal RNA (rRNA) from RNA samples.

rRNA Depletion: A Key Step for RNA-Seq Efficiency

Ribosomal RNAs (rRNAs) dominate cellular RNA, making up over 90% of total RNA in most cells. As the core components of ribosomes, rRNAs are essential for translating mRNAs into proteins.

However, in RNA-Seq projects, this abundance poses a challenge—rRNAs can overshadow mRNAs and other low-abundance transcripts, which typically account for only 2-3% of the sequencing output.

Why Remove rRNA?
rRNA removal is a critical step in RNA-Seq workflows, offering two key benefits:

  1. Improved Transcript Coverage: Enables detection of rare and less abundant RNA species.
  2. Cost-Efficiency: Maximizes sequencing data focused on transcripts of interest, reducing wasted reads.

Optimize your RNA-Seq results by starting with effective rRNA depletion!