Custom riboPOOLs

In RNA-Seq, the presence of highly expressed transcripts which may have limited biological relevance, such as ribosomal RNAs (rRNA), can overshadow the identification of more valuable low-abundance transcripts. This issue is particularly pronounced when confronted with sample types lacking accessible rRNA depletion solutions.

Addressing this issue custom riboPOOLs offer tailored solutions for eliminating unwanted rRNAs and other abundant RNAs from any organism. By designing complex pools of biotinylated DNA probes, specifically designed to hybridize and remove ribosomal RNAs (rRNA) from RNA samples, we can create riboPOOLs for:

  • Any species or mixture of RNA (e.g., seawater samples)

  • High quality to strongly degraded RNA (i.e., FFPE samples)

  • Standard RNA-Seq or Ribosome Profiling (Ribo-Seq) samples

Depletion of rRNAs with riboPOOLs can be easily incorporated into established laboratory protocols, and is compatible with many downstream applications, including short and long-read RNA sequencing and Ribosome Profiling (Ribo-Seq).

How to order your custom rRNA depletion kit?

Order a customized ribosomal RNA depletion kits (custom riboPOOLs) for your target species.

By following these steps:

for Ribo-Seq for any species

for RNA-Seq for any species

What customers say

"We are doing a lot of ribosome profiling experiments - mainly using mouse tissues and cell lines - and were in need of a ribosomal RNA depletion tool that was robust, economical, and adaptable. Initially, we asked siTOOLs Biotech to design specific antisense probes against the most abundant rRNA contaminants that we tended to see in our ribosome profiling data, and that severely reduced the amount of usable reads. The probes reduced the ribosomal RNAs they were designed for very well, however (and probably as to be expected) other rRNA contaminants subsequently became predominant and we needed a more uniform, rational tool that would cover all rRNA contaminants. Hence, siTOOLs Biotech designed and produced a ribosomal RNA depletion kit depleting both extremely abundant rRNA contaminants and less abundant ones. We are very happy with the support, reactivity, and overall scientific expertise and knowledge of the siTOOLs team, which allowed us to have a great tool for a variety of experiments ongoing in the lab."

Dr. David Gatfield (University of Lausanne, Center for Integrative Genomics (CIG))

Publication: Bhat et al. (2021) Science