Blood Parasite
rRNA depletion kits
Efficient ribodepletion for blood parasites
The Blood Parasite riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from blood parasites' RNA samples. The product is compatible with most downstream applications, including different next-generation sequencing workflows (both short and long-read sequencing).
Available riboPOOL products and their recommended applications:
| Product | Input RNA Quality | Input RNA | Application |
|---|---|---|---|
| Pan riboPOOL | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| Pan High Input riboPOOL | High Quality (RIN>7) | 5 µg - 30 µg | Standard RNA-seq |
Blood parasites are organisms that inhabit the bloodstream of animals, including humans, and can cause a variety of diseases.
Shop riboPOOLs by application
Pan riboPOOL
rRNA depletion for multiple species
High Input
Pan riboPOOL for large RNA quantities
Sequences covered by Blood Parasite riboPOOLs
| Sequence | Type | Pan | High Input |
|---|---|---|---|
| 28S rRNA | Cytoplasmic | ✓ | ✓ |
| 18S rRNA | Cytoplasmic | ✓ | ✓ |
| 5.8S rRNA | Cytoplasmic | ✓ | ✓ |
| 5S rRNA | Cytoplasmic | ✓ | ✓ |
Performance
The Blood Parasite riboPOOL was shown to consistently remove > 85% of rRNA from total RNA samples of different Leishmania species.
Figure 1 Percentage of rRNA reads of different Leishmania species before and after rRNA depletion with the Blood Parasite riboPOOL. Data kindly provided by David Langlais (McGill University).
Blood Parasite Taxonomic Coverage
The Blood Parasite riboPOOL was designed using the rRNA sequences from these species as templates:
Babesia microti
Babesia ovata
Leishmania amazonensis
Leishmania brasiliensis
Leishmania donovani
Leishmania infantum
Leishmania major
Plasmodium berghei
Plasmodium chabaudi
Plasmodium falciparum
Plasmodium vivax
Toxoplasma cruzi
Toxoplasma gondii
Would you like to know how well the Blood Parasite riboPOOL covers your species of interest? Contact us for a free bioinformatic coverage assessment.
Not finding what you are looking for?
All of our riboPOOLs can be customized or combined with different species to provide individual solutions that fit your experiment. Contact us at info@sitools.de for more information.