Pan-Bacteria riboPOOL
The Pan-Bacteria riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from gram negative and gram positive bacteria RNA samples. The product is compatible with most downstream applications, including different next generation sequencing workflows (both short and long-read sequencing).
The Pan-Bacteria riboPOOL for bacterial rRNA depletion is optimized for high-quality and intact (RIN > 7) RNA samples.
The Pan-Bacteria riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 23S rRNA | Cytosolic | Yes |
| 16S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
Several bacteria are considered model organisms in scientific research due to their unique characteristics, genetic tractability, and relevance to various fields of study. For example: Escherichia coli, Bacillus subtilis, and Mycobacterium tuberculosis.
Benefits of using riboPOOLs for multiple species rRNA depletion
- Enzyme-free, fast & easy workflow
- Compatible with most downstream applications
- Wide range of input RNA amounts
- Possibility of combining with other riboPOOLs
- Compatible with lab automated workflows
Pan-Bacteria riboPOOL Performance
The Pan-Bacteria riboPOOL efficiently depleted > 90% of rRNA across Bacteria.
Figure 1 Remaining rRNA expression of different bacteria species after ribodepletion with Pan-Bacteria riboPOOL. Expression values shown are normalized against a non-depleted total RNA sample.
Pan-Bacteria Taxonomic Coverage
The Pan-Bacteria riboPOOL is designed for efficient ribodepletion across Bacteria. Pan-Bacteria riboPOOL probes have been mapped against rRNA sequences of more than 12500 Bacteria species to ensure uniform coverage across the taxon.
Would you like to know how well the Pan-Bacteria riboPOOL covers your Bacteria species of interest? Contact us for a free bioinformatic coverage assessment. Click here for more information on how our Pan-riboPOOLs are designed.