Pan-Mammal riboPOOL
The Pan-Mammal riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from mammal RNA samples. The product is compatible with most downstream applications, including different next generation sequencing workflows (both short and long-read sequencing).
The Pan-Mammal riboPOOL is optimized for high-quality and intact (RIN > 7) RNA samples.
The Pan-Mammal riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | Yes |
| 12S rRNA | Mitochondrial | Yes |
Mammals represent a diverse class of warm-blooded vertebrates characterized by traits like mammary glands, fur or hair, and a neocortex in the brain. This highly biodiverse group showcases remarkable adaptability and serves as essential models in scientific research due to their physiological, genetic, and behavioral similarities to humans.
Benefits of using riboPOOLs for multiple species rRNA depletion
- Enzyme-free, fast & easy workflow
- Compatible with most downstream applications
- Wide range of input RNA amounts
- Possibility of combining with other riboPOOLs
- Compatible with lab automated workflows
Pan-Mammal riboPOOL Performance
The Pan-Mammal riboPOOL consistently removes > 90% of rRNA from total RNA samples originating from different mammalian species (Figure 1). The riboPOOL is designed to target different conserved regions of each rRNA, ensuring a high wide taxonomic coverage across the clade (Figure 2, Figure 3).
Figure 1 Percentage of rRNA reads of different mammal species before and after rRNA depletion with the Pan-Mammal riboPOOL.
Pan-Mammal Taxonomic Coverage
The Pan-Mammal riboPOOL is designed for efficient ribodepletion in mammals (class Mammalia). Pan-Mammal riboPOOL probes have been mapped against rRNA sequences of 75 mammal species to ensure uniform coverage across the phylum.
Would you like to know how well the Pan-Mammal riboPOOL covers your mammal species of interest? Contact us for a free bioinformatic coverage assessment. Click here for more information on how our Pan-riboPOOLs are designed.