Pan-Sponge
rRNA depletion kit
Efficient ribodepletion for sponges
The Pan-Sponge riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from sponge RNA samples. The product is compatible with most downstream applications, including different next generation sequencing workflows (both short and long-read sequencing).
The Pan-Sponge riboPOOL is optimized for high-quality and intact (RIN > 7) RNA samples.
The Pan-Sponge riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytoplasmic | Yes |
| 18S rRNA | Cytoplasmic | Yes |
| 5.8S rRNA | Cytoplasmic | Yes |
| 5S rRNA | Cytoplasmic | No |
| 16S rRNA | Mitochondrial | Yes |
| 12S rRNA | Mitochondrial | Yes |
Sponges are crucial for maintaining the health of marine ecosystems through their filter-feeding activities. Molecular biology studies help understand the genetic responses of sponges to environmental changes and pollution, which can have broader ecological implications.
The Pan-Sponge riboPOOL was designed using the rRNA sequences from these species as templates:
Amphimedon queenslandica
Ephydatia fluviatilis
Lendenfeldia chondrodes
Spongilla lacustris
Tethya spp.
Benefits of using riboPOOLs for multiple species rRNA depletion
- Enzyme-free, fast & easy workflow
- Compatible with most downstream applications
- Wide range of input RNA amounts
- Possibility of combining with other riboPOOLs
- Compatible with lab automated workflows
Performance
The Pan-Sponge riboPOOL was shown to consistently remove > 95% of rRNA from L. chondrodes total RNA samples.
Figure 1 Percentage of rRNA reads in two samples of Lendenfeldia chondrodes total RNA after rRNA depletion with the Pan-Sponge riboPOOL.