Acanthamoeba castellanii riboPOOL for ribosome profiling
The Acanthamoeba castellanii Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) of Acanthamoeba castellanii during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
The Acanthamoeba castellanii riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 12S rRNA | Mitochondrial | No |
| 16S rRNA | Mitochondrial | No |
Acanthamoeba species can act as opportunistic pathogens, causing infections in humans and other organisms. Molecular studies explore the genetic and cellular aspects of host-parasite interactions, aiding in understanding pathogenic mechanisms and immune responses.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species