Caenorhabditis elegans riboPOOL for ribosome profiling
The Caenorhabditis elegans Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from Caenorhabditis elegans during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
For high-quality and intact (RIN > 7) RNA samples, see Caenorhabditis elegans riboPOOL and for depletion of rRNA in FFPE/degraded sample, see Caenorhabditis elegans degraded RNA riboPOOL.
The Caenorhabditis elegans riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 26S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
The ease of genetic manipulation in Caenorhabditis elegans has facilitated groundbreaking studies on gene regulation, RNA interference (RNAi), and functional genomics. It has been pivotal in identifying genes that control essential biological processes.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species