Danio rerio Ribo-Seq
rRNA depletion kit
Efficient ribodepletion for Zebrafish
The Danio rerio Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) during Ribo-Seq experiments. It’s also fully compatible with total RNA-Seq samples, making it a versatile option for project including both Ribo-Seq and RNA-Seq.
Ribodepletion with riboPOOLs can be easily integrated into Ribo-Seq protocols without requiring modifications to established workflows.
If you're only working with total RNA-Seq check out our Danio rerio riboPOOL(for intact RNA) and Danio rerio intact/degraded RNA riboPOOL (for intact and degraded RNA).
The Danio rerio Ribo-Seq riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytoplasmic | Yes |
| 18S rRNA | Cytoplasmic | Yes |
| 5.8S rRNA | Cytoplasmic | Yes |
| 5S rRNA | Cytoplasmic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Zebrafish are amenable to genetic manipulation, including transgenesis and CRISPR/Cas9-mediated genome editing. Researchers utilize these tools to introduce or knock out specific genes, unraveling their roles in development, disease, and other physiological processes.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species