Drosophila melanogaster riboPOOL for ribosome profiling
The Drosophila melanogaster Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) Drosophila melanogaster during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
For high-quality and intact (RIN > 7) RNA samples, see Drosophila melanogaster riboPOOL and for depletion of rRNA in FFPE/degraded sample, see Drosophila melanogaster degraded RNA riboPOOL.
The Drosophila melanogaster riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 2S rRNA | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Drosophila has been pivotal in the discovery and understanding of RNAi. Molecular studies involving RNAi in fruit flies have provided a foundation for harnessing this mechanism for gene silencing in various organisms.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species