Escherichia coli riboPOOL for ribosome profiling
The Escherichia coli Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from Escherichia coli during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
For high-quality and intact (RIN > 7) RNA samples, see Escherichia coli riboPOOL and for depletion of rRNA in FFPE/degraded sample, see Escherichia coli degraded RNA riboPOOL.
The Escherichia coli Ribo-Seq riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 23S rRNA | Cytosolic | Yes |
| 16S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
Escherichia coli, is a gram-negative, rod-shaped bacterium that plays a dual role in the realm of microbiology. While some strains of E. coli are harmless and part of the normal gut microbiota of humans and animals, others can cause infections, ranging from mild gastroenteritis to severe illnesses.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species