Human riboPOOL for ribosome profiling
The Human Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from human during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
For high-quality and intact (RIN > 7) RNA samples, see Human riboPOOL and for depletion of rRNA in FFPE/degraded sample, see Human FFPE/Degraded RNA riboPOOL.
The human riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Through ribosome profiling, scientists gain invaluable insights into the intricacies of protein synthesis, uncovering details about translational efficiency, regulatory elements, and uncovering potential therapeutic targets within the human genome.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species