Human Ribo-Seq
rRNA depletion kit
Efficient ribodepletion for human
The Human Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) during Ribo-Seq experiments. It’s also fully compatible with total RNA-Seq samples, making it a versatile option for project including both Ribo-Seq and RNA-Seq.
Ribodepletion with riboPOOLs can be easily integrated into Ribo-Seq protocols without requiring modifications to established workflows.
If you're only working with total RNA-Seq check out our Human riboPOOL (for intact RNA) and Human intact/degraded RNA riboPOOL (for intact and degraded RNA).
The human Ribo-Seq riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytoplasmic | Yes |
| 18S rRNA | Cytoplasmic | Yes |
| 5.8S rRNA | Cytoplasmic | Yes |
| 5S rRNA | Cytoplasmic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Through ribosome profiling, scientists gain invaluable insights into the intricacies of protein synthesis, uncovering details about translational efficiency, regulatory elements, and uncovering potential therapeutic targets within the human genome.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species