Leishmania mexicana riboPOOL for ribosome profiling

The Leishmania mexicana Ribo-Seq riboPOOL is designed to efficiently deplete ribosomal RNA (rRNA) from Leishmania mexicana during Ribo-Seq sample preparation.

Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols. 

 

The Leishmania mexicana riboPOOL covers the following sequences:

Sequence Type Covered
28S-α rRNA Cytosolic Yes
28S-β rRNA Cytosolic Yes
28S-γ rRNA (M1) Cytosolic Yes
28S-δ rRNA (M2) Cytosolic Yes
28S-ε rRNA (M4) Cytosolic Yes
28S-ζ rRNA (M6) Cytosolic Yes
18S  rRNA Cytosolic Yes
5.8S  rRNA (M3) Cytosolic Yes
5S rRNA (M5) Cytosolic Yes
16S  rRNA Mitochondrial No
12S  rRNA Mitochondrial No

 

Leishmania mexicana is a protozoan parasite transmitted through the bite of infected sandflies. Known to cause cutaneous leishmaniasis, a skin infection affecting humans and other mammals. 

Benefits

  • Enzyme-free, fast & easy workflow
  • Compatible with most downstream applications
  • Wide range of input RNA amounts
  • Possibility of combining with other riboPOOLs
  • Compatible with lab automated workflows

Available Formats

riboPOOLs kits with cleanUP module

riboPOOL Probes Only