Leishmania mexicana riboPOOL for ribosome profiling
The Leishmania mexicana Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from Leishmania mexicana (parasite) during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
The Leishmania mexicana riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S-α rRNA | Cytosolic | Yes |
| 28S-β rRNA | Cytosolic | Yes |
| 28S-γ rRNA (M1) | Cytosolic | Yes |
| 28S-δ rRNA (M2) | Cytosolic | Yes |
| 28S-ε rRNA (M4) | Cytosolic | Yes |
| 28S-ζ rRNA (M6) | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA (M3) | Cytosolic | Yes |
| 5S rRNA (M5) | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Leishmania mexicana is a protozoan parasite transmitted through the bite of infected sandflies. Known to cause cutaneous leishmaniasis, a skin infection affecting humans and other mammals.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species