Mouse-Rat Ribo-Seq
rRNA depletion kit
Efficient ribodepletion for mouse and rat
The Mouse-Rat Ribo-Seq riboPOOLis designed to efficiently remove ribosomal RNA (rRNA) during Ribo-Seq experiments. It’s also fully compatible with total RNA-Seq samples, making it a versatile option for project including both Ribo-Seq and RNA-Seq.
Ribodepletion with riboPOOLs can be easily integrated into Ribo-Seq protocols without requiring modifications to established workflows.
If you're only working with total RNA-Seq check out our Mouse-Rat riboPOOL (for intact RNA) and Mouse-Rat intact/degraded RNA riboPOOL (for intact and degraded RNA).
The Mouse-Rat Ribo-Seq riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytoplasmic | Yes |
| 18S rRNA | Cytoplasmic | Yes |
| 5.8S rRNA | Cytoplasmic | Yes |
| 5S rRNA | Cytoplasmic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Ribosome profiling studies in mice and rats involve capturing snapshots of the ribosome-bound mRNA fragments, providing insights into translation dynamics and identifying actively translated genes. These studies help understand gene expression regulation, translational control mechanisms, and protein synthesis rates in these model organisms.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species