Mouse-Rat Ribo-Seq riboPOOL
The Mouse-Rat Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from mouse and rat during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
For high-quality and intact (RIN > 7) RNA samples, see Mouse-Rat riboPOOL and for depletion of rRNA in FFPE/degraded sample, see Mouse-Rat degraded RNA riboPOOL.
The Mouse-Rat Ribo-Seq riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Ribosome profiling studies in mice and rats involve capturing snapshots of the ribosome-bound mRNA fragments, providing insights into translation dynamics and identifying actively translated genes. These studies help understand gene expression regulation, translational control mechanisms, and protein synthesis rates in these model organisms.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species