Saccharomyces cerevisiae riboPOOL for ribosome profiling
The Saccharomyces cerevisiae Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from Saccharomyces cerevisiae (yeast) during Ribo-Seq sample preparation.
Ribodepletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
For high-quality and intact (RIN > 7) RNA samples, see Saccharomyces cerevisiae riboPOOL and for depletion of rRNA in FFPE/degraded sample, see Saccharomyces cerevisiae degraded RNA riboPOOL.
The Saccharomyces cerevisiae riboPOOL (yeast rRNA depletion kit) covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Research involving Saccharomyces cerevisiae and ribosome profiling has unveiled intricate details of translation dynamics within cells. By mapping ribosome positions along messenger RNAs, researchers have deciphered key aspects of translation efficiency, ribosome pausing, and the impact of specific sequence features on protein synthesis.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species