Toxoplasma gondii Ribo-Seq
rRNA depletion kit
Efficient ribodepletion for Toxoplasma gondii
The Toxoplasma gondii Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) during Ribo-Seq experiments. It’s also fully compatible with total RNA-Seq samples, making it a versatile option for project including both Ribo-Seq and RNA-Seq.
Ribodepletion with riboPOOLs can be easily integrated into Ribo-Seq protocols without requiring modifications to established workflows.
If you're only working with total RNA-Seq check out our Toxoplasma gondii intact and degraded RNA riboPOOL (for intact and degraded RNA).
The Toxoplasma gondii Ribo-Seq riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 26S rRNA | Cytoplasmic | Yes |
| 18S rRNA | Cytoplasmic | Yes |
| 5.8S rRNA | Cytoplasmic | Yes |
| 5S rRNA | Cytoplasmic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Toxoplasma gondii is an obligate intracellular protozoan parasite that infects warm-blooded animals, including humans, causing toxoplasmosis. Researchers investigate various aspects of its molecular biology, including its lifecycle, genetic makeup, host-parasite interactions, and mechanisms of infection and pathogenesis.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species
Performance
The Toxoplasma gondii Ribo-Seq riboPOOL was shown to consistently remove > 95% of rRNA from T. gondii Ribo-Seq samples.
Figure 1 Percentage of human and Toxoplasma gondii rRNA reads in Ribo-Seq samples depleted with Human + Toxoplasma gondii riboPOOLs and competitor product. Percentages on the left refer to the sum of human and T. gondii rRNA reads. Data kindly provided by Michael Holmes (Indiana University).