Trypanosoma brucei riboPOOL for ribosome profiling
The Trypanosoma brucei Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from Trypanosoma brucei (parasite) during Ribo-Seq sample preparation.
Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols.
The Trypanosoma brucei riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S-α rRNA | Cytosolic | Yes |
| 28S-β rRNA | Cytosolic | Yes |
| 28S-γ rRNA (M1) | Cytosolic | Yes |
| 28S-δ rRNA (M2) | Cytosolic | Yes |
| 28S-ε rRNA (M4) | Cytosolic | Yes |
| 28S-ζ rRNA (M6) | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA (M3) | Cytosolic | Yes |
| 5S rRNA (M5) | Cytosolic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Trypanosoma brucei is a single-celled parasite that causes African trypanosomiasis, also known as sleeping sickness in humans, which is transmitted by the tsetse fly. It's a focus of extensive research due to its impact on public health and agriculture, with studies aiming to understand its biology, transmission, host interactions, and potential treatments for the diseases it causes.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species