Trypanosoma brucei riboPOOL for ribosome profiling

The Trypanosoma brucei Ribo-Seq riboPOOL is designed to efficiently deplete ribosomal RNA (rRNA) from Trypanosoma brucei during Ribo-Seq sample preparation.

Depletion with Ribo-Seq riboPOOLs can be readily integrated into Ribo-Seq workflows without the need of modifications to established protocols. 

 

The Trypanosoma brucei riboPOOL covers the following sequences:

Sequence Type Covered
28S-α rRNA Cytosolic Yes
28S-β rRNA Cytosolic Yes
28S-γ rRNA (M1) Cytosolic Yes
28S-δ rRNA (M2) Cytosolic Yes
28S-ε rRNA (M4) Cytosolic Yes
28S-ζ rRNA (M6) Cytosolic Yes
18S  rRNA Cytosolic Yes
5.8S  rRNA (M3) Cytosolic Yes
5S rRNA (M5) Cytosolic Yes
16S  rRNA Mitochondrial No
12S  rRNA Mitochondrial No

 

Trypanosoma brucei is a single-celled parasite that causes African trypanosomiasis, also known as sleeping sickness in humans, which is transmitted by the tsetse fly. It's a focus of extensive research due to its impact on public health and agriculture, with studies aiming to understand its biology, transmission, host interactions, and potential treatments for the diseases it causes.

Benefits

  • Enzyme-free, fast & easy workflow
  • Compatible with most downstream applications
  • Wide range of input RNA amounts
  • Possibility of combining with other riboPOOLs
  • Compatible with lab automated workflows

Available Formats

riboPOOLs kits with cleanUP module

riboPOOL Probes Only