Trypanosoma brucei Ribo-Seq
rRNA depletion kit
Efficient ribodepletion for Trypanosoma brucei
The Trypanosoma brucei Ribo-Seq riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) during Ribo-Seq experiments. It’s also fully compatible with total RNA-Seq samples, making it a versatile option for project including both Ribo-Seq and RNA-Seq.
Ribodepletion with riboPOOLs can be easily integrated into Ribo-Seq protocols without requiring modifications to established workflows.
If you're only working with total RNA-Seq check out our Trypanosoma brucei intact and degraded RNA riboPOOL (for intact and degraded RNA).
The Trypanosoma brucei Ribo-Seq riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S-α rRNA | Cytoplasmic | Yes |
| 28S-β rRNA | Cytoplasmic | Yes |
| 28S-γ rRNA (M1) | Cytoplasmic | Yes |
| 28S-δ rRNA (M2) | Cytoplasmic | Yes |
| 28S-ε rRNA (M4) | Cytoplasmic | Yes |
| 28S-ζ rRNA (M6) | Cytoplasmic | Yes |
| 18S rRNA | Cytoplasmic | Yes |
| 5.8S rRNA (M3) | Cytoplasmic | Yes |
| 5S rRNA (M5) | Cytoplasmic | Yes |
| 16S rRNA | Mitochondrial | No |
| 12S rRNA | Mitochondrial | No |
Trypanosoma brucei is a single-celled parasite that causes African trypanosomiasis, also known as sleeping sickness in humans, which is transmitted by the tsetse fly. It's a focus of extensive research due to its impact on public health and agriculture, with studies aiming to understand its biology, transmission, host interactions, and potential treatments for the diseases it causes.
Benefits of using riboPOOLs for ribosome profiling
- Enhance the cost-efficiency of sequencing projects
- Facilitate the quantification of translation of rare transcripts
- Customizable rRNA removal solution for any species