Caenorhabditis elegans
rRNA depletion kits
Efficient ribodepletion for Caenorhabditis elegans
Our Caenorhabditis elegans riboPOOLs efficiently remove rRNA from Caenorhabditis elegans RNA samples. The product is compatible with most downstream applications, including different NGS workflows (both short and long-read sequencing).
Available riboPOOL products and their recommended applications:
| Product | Input RNA Quality | Input RNA | Application |
|---|---|---|---|
| Intact RNA | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| Intact/degraded RNA | Any quality | 10 ng- 3 µg | Standard RNA-seq |
| Ribosome Profiling | Any quality | 10 ng- 3 µg | Ribo-seq |
| High Input RNA | High Quality (RIN>7) | 5 µg - 30 µg | Standard RNA-seq |
The ease of genetic manipulation in Caenorhabditis elegans has facilitated groundbreaking studies on gene regulation, RNA interference (RNAi), and functional genomics. It has been pivotal in identifying genes that control essential biological processes.
Shop riboPOOLs by application
Intact RNA
riboPOOL for high-quality samples
Intact and Degraded
riboPOOL for any-quality samples
Ribosome Profiling
riboPOOL for ribo-seq applications
High Input
riboPOOL for large RNA quantities
Not finding what you are looking for?
All of our riboPOOLs can be customized or combined with different species to provide individual solutions that fit your experiment. Contact us at info@sitools.de for more information.
Sequences covered by Caenorhabditis elegans riboPOOLs
| Sequence | Type | Intact RNA | Intact/Degraded RNA | Ribosome Profiling | High Input |
|---|---|---|---|---|---|
| 28S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ |
| 18S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ |
| 5.8S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ |
| 5S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ |
| 16S rRNA | Mitochondrial | ✓ | ✕ | ✕ | ✓ |
| 12S rRNA | Mitochondrial | ✓ | ✕ | ✕ | ✓ |
Performance
Figure 1 Bioanalyzer profiles of Caenorhabditis elegans total RNA before and after rRNA depletion with the C. elegans riboPOOL for intact RNA.
Figure 2 Percentage of rRNA reads in samples of Caenorhabditis elegans total RNA before and after rRNA depletion with the C. elegans riboPOOL for intact RNA.