Deroceras laeve
rRNA depletion kits
Efficient ribodepletion for marsh slugs
The Deroceras laeve riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from Deroceras laeve (marsh slug) RNA samples. The product is compatible with most downstream applications, including different next generation sequencing workflows (both short and long-read sequencing).
Available riboPOOL products and their recommended applications:
| Product | Input RNA Quality | Input RNA | Application |
|---|---|---|---|
| Intact riboPOOL | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| High Input riboPOOL | High Quality (RIN>7) | 5 µg - 30 µg | Standard RNA-seq |
The marsh slug, Deroceras laeve, is a terrestrial gastropod found in various habitats, including wetlands and gardens.
Shop riboPOOLs by application
Intact RNA
riboPOOL for high-quality samples
High Input
riboPOOL for large RNA quantities
Not finding what you are looking for?
All of our riboPOOLs can be customized or combined with different species to provide individual solutions that fit your experiment. Contact us at info@sitools.de for more information.
Sequences covered by Deroceras laeve riboPOOLs
| Sequence | Type | Intact RNA | High Input |
|---|---|---|---|
| 28S rRNA | Cytoplasmic | ✓ | ✓ |
| 18S rRNA | Cytoplasmic | ✓ | ✓ |
| 5.8S rRNA | Cytoplasmic | ✓ | ✓ |
| 5S rRNA | Cytoplasmic | ✓ | ✓ |
Performance
The Deroceras laeve riboPOOL was shown to consistently remove > 90% of rRNA from D. laeve total RNA samples and provide rRNA depletion level equivalent to Poly-A selection.
Figure 1 Percentage of rRNA reads in three samples of Deroceras laeve total RNA before and after rRNA depletion with the Deroceras laeve riboPOOL or Poly-A selection. Data kindly provided by Dr. Petr Svoboda (Institute of Molecular Geneticsof the Czech Academy of Sciences, Czech Republic).