Pan-Sponge
rRNA depletion kits
Efficient ribodepletion for sponges
The Pan-Sponge riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from sponge RNA samples. The product is compatible with most downstream applications, including different next generation sequencing workflows (both short and long-read sequencing).
Available riboPOOL products and their recommended applications:
| Product | Input RNA Quality | Input RNA | Application |
|---|---|---|---|
| Pan riboPOOL | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| Pan High Input riboPOOL | High Quality (RIN>7) | 5 µg - 30 µg | Standard RNA-seq |
Sponges are important for marine ecosystem health. Molecular studies help reveal their genetic responses to environmental change and pollution, with broader ecological implications.
Shop riboPOOLs by application
Pan riboPOOL
rRNA depletion for multiple species
High Input
Pan riboPOOL for large RNA quantities
Sequences covered by Pan-Sponge riboPOOLs
| Sequence | Type | Pan | High Input |
|---|---|---|---|
| 28S rRNA | Cytoplasmic | ✓ | ✓ |
| 18S rRNA | Cytoplasmic | ✓ | ✓ |
| 5.8S rRNA | Cytoplasmic | ✓ | ✓ |
| 5S rRNA | Cytoplasmic | ✕ | ✕ |
| 16S rRNA | Mitochondrial | ✓ | ✓ |
| 12S rRNA | Mitochondrial | ✓ | ✓ |
Performance
The Pan-Sponge riboPOOL was shown to consistently remove > 95% of rRNA from L. chondrodes total RNA samples.
Figure 1 Percentage of rRNA reads in two samples of Lendenfeldia chondrodes total RNA after rRNA depletion with the Pan-Sponge riboPOOL.
Pan-Sponge Taxonomic Coverage
The Pan-Sponge riboPOOL was designed using the rRNA sequences from these species as templates:
Amphimedon queenslandica
Ephydatia fluviatilis
Lendenfeldia chondrodes
Spongilla lacustris
Tethya spp.
Would you like to know how well the Pan-Sponge riboPOOL covers your Sponge species of interest? Contact us for a free bioinformatic coverage assessment.
Not finding what you are looking for?
All of our riboPOOLs can be customized or combined with different species to provide individual solutions that fit your experiment. Contact us at info@sitools.de for more information.