Pinus sylvestris
rRNA depletion kits
Efficient ribodepletion for Pinus sylvestris
The Pinus sylvestris riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) in Pinus sylvestris. The product is compatible with most downstream applications, including different next generation sequencing workflows (both short and long-read sequencing).
Available riboPOOL products and their recommended applications:
| Product | Input RNA Quality | Input RNA | Application |
|---|---|---|---|
| Intact riboPOOL | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| High Input riboPOOL | High Quality (RIN>7) | 5 µg - 30 µg | Standard RNA-seq |
Pinus sylvestris, commonly known as Scots Pine, is a widely distributed pine species in Europe and Asia, renowned for its adaptability to various climates.
Shop riboPOOLs by application
Intact RNA
rRNA depletion for multiple species
High Input
riboPOOL for large RNA quantities
Not finding what you are looking for?
All of our riboPOOLs can be customized or combined with different species to provide individual solutions that fit your experiment. Contact us at info@sitools.de for more information.
Sequences covered by Pinus sylvestris riboPOOLs
| Sequence | Type | Intact RNA | High Input |
|---|---|---|---|
| 28S rRNA | Cytoplasmic | ✓ | ✓ |
| 18S rRNA | Cytoplasmic | ✓ | ✓ |
| 5.8S rRNA | Cytoplasmic | ✓ | ✓ |
| 5S rRNA | Cytoplasmic | ✓ | ✓ |
| 26S rRNA | Mitochondrial | ✓ | ✓ |
| 18S rRNA | Mitochondrial | ✓ | ✓ |
| 5S rRNA | Mitochondrial | ✓ | ✓ |
| 23S rRNA | Plastid | ✓ | ✓ |
| 16S rRNA | Plastid | ✓ | ✓ |
| 5S rRNA | Plastid | ✓ | ✓ |
| 4.5S rRNA | Plastid | ✓ | ✓ |
Performance
The Pinus sylvestris riboPOOL was shown to consistently remove > 95% of rRNA from Pinus sylvestris total RNA samples.
Figure 1 Percentage of eukaryotic and prokaryotic rRNA reads in total RNA samples of Pinus sylvestris depleted with a combination of Pinus sylvestris and Pan-Bacteria riboPOOLs. Percentages on the left refer to the sum of eukaryotic and prokaryotic rRNA reads. Data kindly provided by Andreas Schneider (Swedish University of Agricultural Sciences).