Escherichia coli
rRNA depletion kits
Efficient ribodepletion for E. coli
Our Escherichia coli riboPOOLs efficiently remove rRNA from E. coli RNA samples. The product is compatible with most downstream applications, including different NGS workflows (both short and long-read sequencing).
Available riboPOOL products and their recommended applications:
| Product | Input RNA Quality | Input RNA | Application |
|---|---|---|---|
| Intact RNA | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| Intact/degraded RNA | Any quality | 10 ng- 3 µg | Standard RNA-seq |
| Ribosome Profiling | Any quality | 10 ng- 3 µg | Ribo-seq |
| High Input RNA | High Quality (RIN>7) | 5 µg - 30 µg | Standard RNA-seq |
| SsrA depletion kit | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| tRNA depletion kit | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
E. coli is a gram-negative bacterium that includes both harmless and pathogenic strains. While many are part of the normal gut microbiota of humans and animals, others can cause infections ranging from mild gastroenteritis to severe disease.
Shop riboPOOLs by application
Intact RNA
riboPOOL for high-quality samples
SsrA Kit
Depletion of SsrA (tmRNA)
Intact and Degraded
riboPOOL for any-quality samples
tRNA Kit
Depletion of tRNA
Ribosome Profiling
riboPOOL for ribo-seq applications
High Input
riboPOOL for large RNA quantities
Not finding what you are looking for?
All of our riboPOOLs can be customized or combined with different species to provide individual solutions that fit your experiment. Contact us at info@sitools.de for more information.
Sequences covered by Escherichia coli riboPOOLs
| Sequence | Type | Intact RNA | Intact/Degraded RNA | Ribosome Profiling | High Input | SsrA Kit | tRNA Kit |
|---|---|---|---|---|---|---|---|
| 23S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ | ✕ | ✕ |
| 16S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ | ✕ | ✕ |
| 5S rRNA | Cytoplasmic | ✓ | ✓ | ✓ | ✓ | ✕ | ✕ |
| SsrA (tmRNA) | Cytoplasmic | ✕ | ✕ | ✕ | ✕ | ✓ | ✕ |
| tRNA | Cytoplasmic | ✕ | ✕ | ✕ | ✕ | ✕ | ✓ |
Performance
Figure 1 Bioanalyzer profiles of Escherichia coli total RNA before and after depletion with the Escherichia coli riboPOOL for intact RNA.
Figure 2 Percentage of rRNA reads in samples of Escherichia coli total RNA before and after rRNA depletion with the Escherichia coli riboPOOL for intact RNA.