Human riboPOOL
The Human riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from human RNA samples. The product is compatible with most downstream applications, including different next generation sequencing workflows (both short and long-read sequencing).
The Human riboPOOL is optimized for high-quality and intact (RIN > 7) RNA samples. For depletion of rRNA in FFPE/degraded sample, see Human FFPE/Degraded RNA riboPOOL.
Working on ribosome profiling? Check our Human Ribo-Seq riboPOOL.
The Human riboPOOL covers the following sequences:
| Sequence | Type | Covered |
|---|---|---|
| 28S rRNA | Cytosolic | Yes |
| 18S rRNA | Cytosolic | Yes |
| 5.8S rRNA | Cytosolic | Yes |
| 5S rRNA | Cytosolic | Yes |
| 12S rRNA | Mitochondrial | Yes |
| 16S rRNA | Mitochondrial | Yes |
Remarkably, only about 1-2% of the human genome encodes for proteins. The remaining portion, once considered "junk DNA," contains regulatory elements and non-coding regions critical for gene regulation and function.
Performance
Figure 1 Bioanalyzer profiles of Human total RNA before and after depletion with Human riboPOOL.
Benefits of using riboPOOLs for rRNA removal
- Enzyme-free, fast & easy workflow
- Compatible with most downstream applications
- Wide range of input RNA amounts
- Possibility of combining with other riboPOOLs
- Compatible with lab automated workflows