Disrupting lncRNA function with siPOOLs (RNAi), antisense oligos and CRISPR
This blogpost covers methods used in the disruption of lncRNA function. Specifically focusing on RNA interference (with siPOOLs), antisense oligos, and CRISPR approaches. Challenges faced with these approaches are addressed.
Correcting seed-based off-target effects in RNAi screens
Unlocking RNAi screening potential by correcting seed-based off-target effects. While seed correction methods yield minor improvements, challenges persist in interpreting strongest hits. The quest for precise reagents, like high-complexity siPOOLs, remains crucial for reliable results.
