Correcting seed-based off-target effects in RNAi screens
Unlocking RNAi screening potential by correcting seed-based off-target effects. While seed correction methods yield minor improvements, challenges persist in interpreting strongest hits. The quest for precise reagents, like high-complexity siPOOLs, remains crucial for reliable results.
Russian roulette
Discover the best bases to choose for the seed region of a single siRNA. Learn about the potential off-target effects and the challenges of predicting them. Explore a recent study that highlights the toxicity of an siRNA in mice and the modifications that were made to create a non-toxic version.
