Pan-Plant
rRNA depletion kits
Efficient ribodepletion for plants
The Pan-Plant riboPOOL is designed to efficiently remove ribosomal RNA (rRNA) from plants RNA samples. The product is compatible with most downstream applications, including different next-generation sequencing workflows (both short and long-read sequencing).
Available riboPOOL products and their recommended applications:
| Product | Input RNA Quality | Input RNA | Application |
|---|---|---|---|
| Pan riboPOOL | High Quality (RIN>7) | 10 ng- 3 µg | Standard RNA-seq |
| Pan High Input riboPOOL | High Quality (RIN>7) | 5 µg - 30 µg | Standard RNA-seq |
RNA-seq in plants compares gene expression across tissues, developmental stages, and conditions such as stress or disease. Key model plants include Arabidopsis thaliana and Oryza sativa.
Shop riboPOOLs by application
Pan riboPOOL
rRNA depletion for multiple species
High Input
Pan riboPOOL for large RNA quantities
Sequences covered by Pan-Plant riboPOOLs
| Sequence | Type | Pan | High Input |
|---|---|---|---|
| 28S rRNA | Cytoplasmic | ✓ | ✓ |
| 18S rRNA | Cytoplasmic | ✓ | ✓ |
| 5.8S rRNA | Cytoplasmic | ✓ | ✓ |
| 5S rRNA | Cytoplasmic | ✓ | ✓ |
| 26S rRNA | Mitochondrial | ✓ | ✓ |
| 18S rRNA | Mitochondrial | ✓ | ✓ |
| 5S rRNA | Mitochondrial | ✓ | ✓ |
| 23S rRNA | Plastid | ✓ | ✓ |
| 16S rRNA | Plastid | ✓ | ✓ |
| 5S rRNA | Plastid | ✓ | ✓ |
| 4.5S rRNA | Plastid | ✓ | ✓ |
Performance
The Pan-Plant riboPOOL consistently removes > 90% of rRNA from total RNA samples originating from different flowering plants species. The riboPOOL is designed to target different conserved regions of each rRNA, ensuring high wide taxonomic coverage across the group.
Figure 1 Percentage of rRNA reads of different flowering plants after rRNA depletion with Pan-Plant riboPOOL. Data kindly provided by Dr. Bruno Huettl and Dr. Christian Wöhle (Max Planck Genome Centre Cologne, Germany).
Pan-Plant Taxonomic Coverage
The Pan-Plant riboPOOL was designed using the rRNA sequences from these species as templates:
Arabidopsis thaliana
Beta vulgaris
Chenopodium quinoa
Citrus sinensis
Coffea arabica
Cucumis sativus
Cynara cardunculus
Daucus carota
Dendrobium catenatum
Elaeis guineensis
Eucalyptus grandis
Glycine max
Gossypium hirsutum
Helianthus annuus
Jatropha curcas
Malus domestica
Nelumbo nucifera
Nymphaea colorata
Olea europaea
Oryza sativa
Papaver somniferum
Populus trichocarpa
Prunus persica
Setaria italica
Solanum tuberosum
Vitis vinifera
Zea mays
The Pan-Plant riboPOOL is designed for efficient ribodepletion in plants. Pan-Plant riboPOOL probes have been mapped against rRNA sequences of more than 100 plant species to ensure uniform coverage across the taxon.
Would you like to know how well the Pan-plant riboPOOL covers your plant species of interest? Contact us for a free bioinformatic coverage assessment.
Not finding what you are looking for?
All of our riboPOOLs can be customized or combined with different species to provide individual solutions that fit your experiment. Contact us at info@sitools.de for more information.