Single-Species riboPOOLs

The best ribodepletion solution for your species

Single-Species riboPOOLs are designed to efficiently target ribosomal RNAs (rRNAs) in a specific organism. Every design aspect of a Single-Species riboPOOL is tailored to its target species. The design workflow takes into account the type and characteristics of the species rRNAs, ensuring the highest specifity between riboPOOL probes and the ribosomal RNA sequences. 

The Single-Species riboPOOLs portfolio includes ribodepletion solutions for more than 60 species, from well-established model organisms (e.g. Escherichia coliArabidopsis thaliana, Drosophila melanogaster, Caenorhabditis elegans) to lesser-known species, and includes options for RNA-Seq (both high quality and degraded RNA) or for ribosome profiling (Ribo-Seq).

If you can't find your species listed below, contact us  to request a custom riboPOOL.

Acetabularia ryukyuensi
ID: 120

Aedes albopictus
ID: 47

Amphimedon queenslandica
ID: 11

Anaeramoeba flamelloides
ID: 95

Argiope bruennichi
ID: 74

Azolla filiculoides
ID: 79

Bacillus subtilis
ID: 12

Bemisia tabaci
ID: 80

Chlamydomonas reinhardtii
ID: 71

Clostridium perfringens
ID: 15

Crassostrea gigas
ID: 63

Cryptococcus neoformans
ID: 84

Drosophila melanogaster
ID: 07

D. melanogaster degraded RNA
ID: 61

Emiliania huxleyi
ID: 33

Equus caballus degraded RNA
ID: 92

Eubacterium limosum
ID: 70

Felis catus degraded RNA
ID: 93

Gallus gallus domesticus
ID: 60

Haloferax volcanii
ID: 16

Human Globin mRNA
ID: 25

Human tRNA
ID: 317

H/M/R degraded RNA
ID: 56

Ixodes scapularis
ID: 30

Nematostella vectensis
ID: 87

Olavius algarvensis
ID: 103

Oryza sativa
ID: 09

Pichia pastoris (fungi)
ID: 19

Saccharomyces cerevisiae
ID: 05

S. cerevisiae degraded RNA
ID: 125

Salmonella enterica
ID: 22

Schistosoma mansoni
ID: 90

S. aureus degraded RNA
ID: 132

Stenotro-phomonas sp.
ID: 23

Strongyloides ratti
ID: 106

Thalassiosira pseudonana
ID: 96

Wolbachia pipientis
ID: 86

Anopheles gambiae
ID: 97

Apis mellifera
ID: 73

Arabidopsis thaliana 
ID: 08

A. thaliana degraded RNA
ID: 123

Caenorhabditis elegans
ID: 39

C. elegans deg. RNA
ID: 62

Caulobacter crescentus
ID: 13

Chinchilla lanigera
ID: 14

Cupriavidus necator
ID: 102

Danio rerio
ID: 10

Danio rerio degraded RNA
ID: 121

Deroceras laeve 
ID: 133

Escherichia coli
ID: 04

E. coli degraded RNA
ID: 122

Escherichia coli tRNA
ID: 334

E. siliculosus degraded RNA
ID: 130

Human
ID: 54

Human Blood
ID: 94

Human Cytoplasm
ID: 98

Human degraded RNA
ID: 57

Leptinotarsa decemlineata
ID: 72

L. mexicana degraded RNA
ID: 126

Mouse-Rat degraded RNA
ID: 58

Mouse tRNA
ID: 322

Pinus sylvestris
ID: 108

Plautia stali
ID: 20

P. pacificus deg. RNA
ID: 127

Pseudomonas aeruginosa
ID: 18

S. pombe
ID: 59

Schmidtea mediterranea
ID: 28

Spodoptera exigua
ID: 85

Staphylococccus aureus
ID: 21

Trypanosoma brucei deg. RNA
ID: 128

Toxoplasma gondii deg. RNA
ID: 129

Ustilago maydis
ID: 24

Varroa destructor
ID: 81

Designing a Single-Species riboPOOL

Step 1. Bioinformatics Design          

Our algorithm scans the rRNA sequences to choose the best probes. Ensuring maximum and optimal rRNA coverage, optimal binding to rRNA sequences, and avoidance of targeting similar genes.

 

Step 2. Laboratory Production 

riboPOOLs are manufactured in accordance with strict Quality Management standards. HPLC is used to refine riboPOOLs to ensure the highest purity levels.

Step 3. Testing the riboPOOL

When ordering a riboPOOL you receive a reliable ribodepletion solution for your target species. riboPOOLs show high rRNA depletion efficacy across species, reaching up to 99% depletion rate.

Reproducible and efficient rRNA removal

riboPOOLs show high rRNA depletion efficacy across species, reaching up to 99%. For strongly degraded RNA samples we offer optimized riboPOOLs to achieve even & efficient rRNA removal of up to 95%. To meet the special requirements of ribosome profiling (Ribo-Seq) we develop dedicated Ribo-Seq riboPOOLs for a growing number of species. 

Figure 1 Bioanalyzer profiles of Human total RNA before and after depletion with Human riboPOOL.

Figure 2 Bioanalyzer profiles of Anopheles gambiae total RNA before and after depletion with the Anopheles gambiae riboPOOL.