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Kinase siRNA Library
Potent & Specific Gene Knockdown for Kinase Proteins
Gene silencing of protein kinases
The Kinase siPOOL Library contains siRNA pools designed to target protein kinases involved in signal transduction, cell-cycle regulation, metabolism, apoptosis and other essential cellular processes.
Each kinase gene is targeted using a complex pool of 30 carefully designed siRNAs. By combining broad transcript coverage with low concentrations of the individual siRNAs, siPOOL technology supports robust and reproducible gene knockdown while minimizing sequence-specific off-target effects, making the library an effective solution for focused and high-throughput RNAi kinase screening.
Kinase siPOOL Library knockdown efficiency
Our Kinase siPOOL Library delivers reliable gene silencing across a broad range of kinase targets.
More than 86% of ramdonly tested kinase siPOOLs reduced target-gene expression by at least 70% knockdown (146 of 170).
In addition, >65% achieved at least 80% knockdown (Fig. 1).
The Kinase siPOOL Library is ideal for:
- High-throughput and focused RNAi kinase screening.
- Investigating kinase functions and cell-signalling pathways.
- Identifying and validating potential therapeutic targets.
- Studying kinase involvement in cancer, inflammatory diseases, metabolic disorders and other pathologies.
Figure 1. Knockdown efficiency of 170 randomly selected human kinase siPOOLs. Target mRNA levels were measured by qRT-PCR 24 hours after transfection with 3 nM siPOOLs in standard cell lines (A549 or MCF7).
Kinase siPOOL Library features and benefits
- Reduced off-target effects: Each of the 30 siRNAs is used at picomolar concentrations, limiting sequence-specific off-target activity.
- Broad transcript coverage: The siRNAs are distributed across the annotated transcripts of each kinase gene, reducing the risk of individual isoforms escaping silencing.
- Robust and reliable knockdown: Combining 30 siRNAs reduces dependence on the performance of any single sequence and supports reproducible results.
- Flexible, screen-ready formats: Available in customizable 96- and 384-well plate layouts with your preferred positive and negative controls.