siPOOLs

Potent & Specific Gene Knock-down by RNAi

Pre-defined siRNA libraries for RNAi screens

siPOOL libraries offer an efficient and cost-effective solution for high-throughput screening of predefined gene families, including kinases, E3 ligases, G protein-coupled receptors (GPCRs), and RNA-binding proteins (RBPs). Designed for accuracy and minimal off-target effects, siPOOL libraries provide reliable gene silencing for your pathway or target-focused research.

Key advantages of using siPOOL libraries for RNAi screening:

  • Low false negative rate: cooperative silencing of 30 siRNAs for reliable target knockdown
  • Low false positive rate: off-target dilution by complex siRNA for highest specificity
  • Efficient screening: screen one reliable pool per target gene
  • Latest genome annotation: target all transcripts of current NCBI release
  • Flexible layout: choose your plate format, layout, and control placement

Available siRNA library formats

Pre-defined libraries Scale
0.1, 0.25, 0.5 or 1 nmol		 Plate format
96 or 384 wells		 Layout
Standard or custom
RBPs (1036 genes)
Kinase (505 genes)
E3 Ligase (647 genes)
GPCRs (393 genes)

Ordering siPOOL libraries

1.  Download library plate layout

2.  Select scale, layout and plate format

3.  Submit to info@sitools.de