Pre-defined siRNA libraries for RNAi screens

siPOOL libraries offer an efficient and cost-effective solution for high-throughput screening of predefined gene families, including kinases, E3 ligases, G protein-coupled receptors (GPCRs), and RNA-binding proteins (RBPs). Designed for accuracy and minimal off-target effects, siPOOL libraries provide reliable gene silencing for your pathway or target-focused research.

Key advantages of using siPOOL libraries for RNAi screening:

  • Low false negative rate: cooperative silencing of 30 siRNAs for reliable target knockdown
  • Low false positive rate: off-target dilution by complex siRNA for highest specificity
  • Efficient screening: screen one reliable pool per target gene
  • Latest genome annotation: target all transcripts of current NCBI release
  • Flexible layout: choose your plate format, layout, and control placement

Available siRNA library formats

Pre-defined libraries Scale
0.1, 0.25, 0.5 or 1 nmol		 Plate format
96 or 384 wells		 Layout
Standard or custom
Kinase (505 genes)
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E3 Ligase (647 genes)
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GPCRs (393 genes)
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RBPs (1036 genes)
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Ordering siPOOL libraries

1.  Download library plate layout

2.  Select scale, layout and plate format

3.  Submit to info@sitools.de