Complex siRNA pools
siPOOLs use low concentrations for each siRNA, reducing off-target effects and delivering reliable phenotypes. Additionally, siPOOLs outperform single siRNAs and mini-pools (fewer than 4 siRNAs) by providing near-optimal silencing of target genes. Learn more on our blog.
Small interfering RNAs (siRNAs) are short double-stranded RNA molecules typically 21 nucleotides in length. Which play an important role in RNA interference (RNAi) by inhibiting the expression of specific genes using complementary nucleotide sequences.
siRNAs can be designed to target specific genes, however single siRNAs can be unpredictable and bind to non-target mRNA sequences (seed effect) leading to off-target effects. Off-target effects can impact the accuracy of RNAi experiments, potentially causing misleading results or unexpected phenotypes. Minimizing these effects is crucial for reliable gene knockdown studies.
siPOOLs: the solution for minimizing off-target effects in RNAi experiments
siPOOLs minimize off-target activity by using the unique approach of complex siRNA pooling. Each siPOOL consists of a specifically designed pool of 30 individual siRNAs, all targeting different sequences within the same target gene. This strategy achieves the following:
- Off-target dilution by very low concentration of each siRNA
- Robust, maximum silencing by cooperative activity of many siRNAs.
Optimized and detailed siRNA design
With defined siRNA sequences within the siPOOL, targeting can be optimized against specific transcript isoforms or closely related genes. Proprietary siRNA design algorithms select the most potent siRNAs based on thermodynamic properties that favor guide strand loading into the RNA-induced silencing complex (RISC). Using latest RefSeq annotations and genome-wide paralogue filtering, siPOOLs are designed for maximum coverage of all targeted transcripts with high specificity.
Available formats
Product | Scale |
Individual siPOOLs | 2 nmol, 5 nmol, 10 nmol, 20 nmol |
siPOOL controls | 2 nmol, 5 nmol, 10 nmol, 20 nmol |
siPOOL libraries | 0.1 nmol, 0.25 nmol, 0.5 nmol, 1 nmol |
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