The final RNAiL?
Explore the debate over RNAi's viability post-CRISPR revelations. Lin et al.'s findings challenge RNAi's efficacy, but nuances suggest RNAi still has value, albeit with precautions against off-target effects
Where's the beef?
Uncover the impact of on- vs. off-target effects in RNAi screening. Analysis of Hasson et al.'s genome-wide screen reveals the prevalence of off-target effects, emphasizing the need for precise siRNA libraries.
Classic Papers Series: Lin et al. show RNAi screen dominated by seed effects
Classic Papers Series: Lin et al. show RNAi screen dominated by seed effects Classic Papers Series: Lin et al. show RNAi screen dominated...
Russian roulette
Discover the best bases to choose for the seed region of a single siRNA. Learn about the potential off-target effects and the challenges of predicting them. Explore a recent study that highlights the toxicity of an siRNA in mice and the modifications that were made to create a non-toxic version.
The beauty of the siPool
Discover the beauty of the siPool strategy. Dilute off-target effects with on-target siRNAs. Like composite faces, flaws are evened out for a beautiful on-target phenotype. Choose the best individual siRNAs for high-complexity siPools.
The nasty, ugly fact of off-target effects
Discover the fascinating history of siRNAs and their impact on gene targeting. Explore the disappointments and lessons learned from siRNA screening.
Don't swallow the fly
There was a PI who screened one s i [RNA], Oh, I don’t know why they screened one s i … siRNA screens have a high false positive rate, due to pervasive off-target effects. Confirming ‘hits’ from single-siRNA screens is a lot of work.
Celebrating 11 years of off-target effects
Celebrating 11 years of off-target effects Celebrating 11 years of off-target effects 2. October 2014 Andrew Walsh This year marks the 11th ...
