The final RNAiL?
Explore the debate over RNAi's viability post-CRISPR revelations. Lin et al.'s findings challenge RNAi's efficacy, but nuances suggest RNAi still has value, albeit with precautions against off-target effects
CRISPR - what can go wrong and how to deal with it
CRISPR's potential pitfalls highlighted: absent phenotypes, inefficient editing, and unexpected results, with strategies for effective management.
Where's the beef?
Uncover the impact of on- vs. off-target effects in RNAi screening. Analysis of Hasson et al.'s genome-wide screen reveals the prevalence of off-target effects, emphasizing the need for precise siRNA libraries.
RNA affinity purification: Tips for optimizing
Optimize RNA Affinity Purification: Key strategies for effective isolation, analysis, and insights into RNA interactions.
The limits of chemical modification
Learn how chemical modifications in siRNAs don't always prevent seed-based off-target effects, as evidenced by Rasmussen et al.'s study on Pik3ca siRNA
Deconvoluted SMARTpools are like a box of chocolates
Exploring RNAi screening, Falkenberg et al. uncover the unpredictability of siRNA effects, highlighting the complexity of gene knockdown studies.
Intronic off-target effects with antisense oligos
Exploring ASOs for RNA interference reveals intronic off-target effects, challenging the specificity of antisense oligonucleotide applications.