Novel anti-cancer mechanism identified by shRNA/siRNA off-target effects
Unveiling a novel anti-cancer mechanism via siRNA/shRNA off-target effects. Putzbach et al.'s study exposes survival genes in cancer cells, shedding light on CD95/CD95L-induced cell death and the role of miRNA-like activity in off-target effects
Little correlation between Dharmacon siGENOME and ON-TARGETplus reagents
Analysis reveals little correlation between Dharmacon siGENOME and ON-TARGETplus, highlighting the complexity of validating RNAi screen hits.
Orthogonal design in software and RNAi screening
Orthogonal design in RNAi screening enhances hit confidence, contrasting with software design principles to minimize systemic interdependencies.
Understanding Gene Networks with Combinatorial Gene Knockdown
Combinatorial gene knockdown with siPOOLs unveils complex gene networks, offering a deeper understanding of gene functions and interactions
"Phenoville" - RNAi & CRISPR Screening Strategies
Comparing RNAi and CRISPR screening strategies in host-virus interactions. RNAi offers broader insights, CRISPR specificity.
CRISPR/Cas9 Screening - The “Copy-Number Effect”
Exploring the `copy number effect` in CRISPR/Cas9 screens for essential genes in cancer cell lines. The phenomenon reveals a challenging source of false positives and negatives, prompting the development of computational methods for correction and emphasizing the need for cautious interpretation and complementary validation techniques.
siRNA vs shRNA - applications and off-targeting
Exploring siRNA vs shRNA for gene silencing: applications, off-target effects, and why choosing the right RNAi method matters for your research.
Unexpected Mutations after CRISPR in vivo editing - post-commentary
Delving into concerns surrounding a Nature Commentary on CRISPR-edited mice, including study size, control choice, Cas9 delivery, and variant validation. Critics emphasize the need for rigorous controls and updated databases to assess off-target effects accurately.
Making sense of siGENOME deconvolution
Deconvolution of Dharmacon siGENOME pools shows surprising results, with low correlation to target genes and significant seed effects.
How reproducible are CRISPR screens?
Exploring the reproducibility of CRISPR screens compared to RNAi screens. While CRISPR gRNAs show more consistent gene inhibition, overlap in top hits between CRISPR screens varies, cautioning against overestimating reliability solely based on assay reproducibility.
